Development and Validation of Method for Simultaneous Estimation of Lamivudine, Zidovudine and Nevirapine

Lamivudine (LMV), Zidovudine (ZDV) and Nevirapine (NVR) are used in combination for treatment of HIV I. The present work deals with method development for simultaneous estimation of these drugs in tablet dosage form by UV spectrophotometry. A new simple, accurate and sensitive method for simultaneous estimation of lamivudine, zidovudine and nevirapine from tablet formulation has been developed. The method employs combination of zero order and first order derivative spectroscopy. Estimation of nevirapine was done in zero order derivative mode at 310 nm where zidovudine and lamivudine do not show any interference. Estimation of zidovudine was done in first order derivative mode at 270 nm where lamivudine and nevirapine shows zero crossing points. Estimation of lamivudine is done in zero order derivative mode at 271 nm by correcting the absorbance interferences due to zidovudine and nevirapine. Absorbance interferences of zidovudine and nevirapine calculated from their absorptivities. The results of analysis validated statistically and recovery studies showed satisfactory results. Thus the method is accurate and reproducible and can be employed for routine analysis of these drugs from tablet dosage forms.