A Simultaneous Estimation of Perindopril and Losartan in Solid Dosage Forms by UV Spectrophotometry

Perindopril and losartan are used in combination for treatment of hypertension. The present work deals with a simple spectrophotometric method development for simultaneous estimation of perindopril (PRL) and losartan (LSN) in two component-formulation. First order derivative spectrophotometric method is adopted to eliminate spectral interference. The method obeys beer’s law in the concentration ranges employed for evaluation. To determine the sampling wave length, 10 mcg/ml of PRL and LSN were scanned from 200-350 nm. The wavelengths were found to be 219 nm for PRL, where LSN showed zero crossing point and 214 nm for LSN, where PRL showed zero crossing point in First order derivative spectroscopy. The recovery studies confirmed accuracy of proposed method and low values of standard deviation confirmed precision of the method. The method is validated as per ICH guidelines.