Asian Journal of Research in Chemistry

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New Visible Spectrophotometric Method for Estimation of Serratiopeptidase from Tablet Formulations


Affiliations
1 Atmiya Institute of Pharmacy, Kalawad Road, Rajkot, Gujarat, India
     

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A simple, economical, precise and rapid visible spectrophotometric method has been developed for the determination of Serratiopeptidase in tablet dosage forms. Developed method is based on the formation of stable colored complex of drug with Biuret reagent. A wavelength maximum was found to be 531 nm. Linearity was observed in the concentration range of 0.5-5 mg/ml. Correlation coefficient was found to be 0.9993. The result of analysis has been validated statistically and also by recovery study.

Keywords

Serratiopeptidase, Visible Spectrophotometric Method, Biuret Reagent.
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  • New Visible Spectrophotometric Method for Estimation of Serratiopeptidase from Tablet Formulations

Abstract Views: 189  |  PDF Views: 0

Authors

Parula Patel
Atmiya Institute of Pharmacy, Kalawad Road, Rajkot, Gujarat, India
Bhavisha Rabadiya
Atmiya Institute of Pharmacy, Kalawad Road, Rajkot, Gujarat, India

Abstract


A simple, economical, precise and rapid visible spectrophotometric method has been developed for the determination of Serratiopeptidase in tablet dosage forms. Developed method is based on the formation of stable colored complex of drug with Biuret reagent. A wavelength maximum was found to be 531 nm. Linearity was observed in the concentration range of 0.5-5 mg/ml. Correlation coefficient was found to be 0.9993. The result of analysis has been validated statistically and also by recovery study.

Keywords


Serratiopeptidase, Visible Spectrophotometric Method, Biuret Reagent.