Visible Extractive Spectrophotometric Estimation of Teicoplanin in Bulk and in Pharmaceutical Formulations

Three simple, accurate, rapid and sensitive methods (A, B and C) have been developed for the estimation of Teicoplanin in pharmaceutical dosage form. The Method A is based on reaction of Teicoplanin with ferric chloride and 1,10-phenanthroline to form a blood red colored chromogen. The Method B is based on reaction of Teicoplanin with ferric chloride 2, 2’ bipyridyl to form blood colored chromogen. The Method C is based on the reduction of Ferric ions of the reagent Ferric chloride to Ferrous ions by the drug, which further in the presence of potassium ferricyanide as oxidizing agent produces blue colored chromogen measured at 700 nm, against reagent blank. These Methods exhibit maximum absorption at 510 nm, 520 nm and 720 nm respectively and obey the Beer’s law in the concentration range of 5-30 mcg/ml, 5-50 mcg/ml and. 1-10 mcg/ml respectively. The Methods have been statistically evaluated and were found to be precise and accurate. The proposed methods are economical and sensitive for the estimation of Teicoplanin in bulk drug and in its formulations.