Asian Journal of Research in Chemistry

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

UV Spectrophotometric Method for the Determination of Gemifloxacin in Bulk and Pharmaceutical Formulation


Affiliations
1 Department of Pharmaceutical Sciences, Andhra University, Visakhapatnam-530003, India
     

   Subscribe/Renew Journal


A simple, sensitive and reproducible spectrophotometric method was developed for the determination of Gemifloxacin (GEMI) in pure and dosage forms. It has absorption maximum at 343 nm and obeys beer’s law in the concentration range 2-20 μg mL-1 .Results of analysis were validated statistically and by recovery studies .The apparent molar absorptivity and sandell’s sensitivity were 1.3862×104 L mol-1 cm-1 and 2.809×10-2 μg cm-2, respectively. The slope and intercept of the equation of the regression line are 0.05370 and -0.00640 respectively. Correlation coefficient was found to be 0.999. This method is successfully employed for the determination of Gemifloxacin in pharmaceutical preparation.
Subscription Login to verify subscription
User
Notifications
Font Size


Abstract Views: 208

PDF Views: 0




  • UV Spectrophotometric Method for the Determination of Gemifloxacin in Bulk and Pharmaceutical Formulation

Abstract Views: 208  |  PDF Views: 0

Authors

S. Ganapathy
Department of Pharmaceutical Sciences, Andhra University, Visakhapatnam-530003, India
D. G. Sankar
Department of Pharmaceutical Sciences, Andhra University, Visakhapatnam-530003, India
Pettla Y. Naidu
Department of Pharmaceutical Sciences, Andhra University, Visakhapatnam-530003, India

Abstract


A simple, sensitive and reproducible spectrophotometric method was developed for the determination of Gemifloxacin (GEMI) in pure and dosage forms. It has absorption maximum at 343 nm and obeys beer’s law in the concentration range 2-20 μg mL-1 .Results of analysis were validated statistically and by recovery studies .The apparent molar absorptivity and sandell’s sensitivity were 1.3862×104 L mol-1 cm-1 and 2.809×10-2 μg cm-2, respectively. The slope and intercept of the equation of the regression line are 0.05370 and -0.00640 respectively. Correlation coefficient was found to be 0.999. This method is successfully employed for the determination of Gemifloxacin in pharmaceutical preparation.