RP-HPLC Determination of Amlodipine Besylate and Atorvastatin in Amlodipine Besilate and Atorvastatin Tablets

A simple, specific, accurate and precise high performance liquid chromatographic method was developed for the determination of Amlodipine and Atorvastatin in Amlodipine besylate and Atorvastatin tablet dosage forms. A Hypersil BDS C₁₈ 5 μm (250×4.6 mm I.D) with column temperature 30°C in isocratic mode, with mobile phase containing Acetonitrile:10 mM Ammonium acetate buffer pH 4.0 (50:50) was used. The flow rate was 1.0 mL/min and effluent was monitored at 238 nm.The retention time of Amlodipine was 2.5 min and for Atorvastatin was 4.7 min. The linearity for Amlodipine and Atorvastatin was in the range 0.02 mg/mL to 0.12 mg/mL. The proposed method is accurate, precise, specific and rapid estimation of Amlodipine and Atorvastatin in Amlodipine besilate and Atorvastatin tablets.