Simultaneous RP-HPLC Method Development and Validation of Cefixime and Ofloxacin in Tablet Dosage Form

A simple, precise, accurate, rapid and reproducible RP-HPLC procedure was developed for simultaneous determination of Cefixime and Ofloxacin in tablet dosage form at a single wavelength. The determination was carried out by using Phenomenox, Gemini C18, Column (50×4.6 mm) with mobile phase used as a combination of acetonitrile:0.05 M Tetra butyl ammonium Hydrogen Sulphate (pH 6.0 with orthophosphoric acid) (35:65). The detection of the combined dosage form was carried out at 290 nm and flow rate set 2.0 ml/min. Linearity obtained in the concentration range of 160 and 240 Cefixime and Ofloxacin with correlation coefficient of 0.9999 and 0.9999 respectively. The result of the analysis were validated statistically and recovery studies confirmed by the accuracy of the proposed method.