Estimation of Gatifloxacin in Tablet Dosage Form by Spectrofluorimetric Method

Three simple and sensitive spectrofluorimetric methods have been developed for the determination of gatifloxacin and its tablet dosage forms. In fluorescence spectrum of gatifloxacin exhibit excitation and emission wavelength at 295 and 498 nm in hydrochloric acid, 295 and 489 nm in acetate buffer pH-4 and 337 and 488 nm, in phthalate buffer pH-4, respectively. The fluorescence intensity for all three methods was found to be linear with the concentration in the range of 200-1000 ng/ml. The % recovery for gatifloxacin was found in the range of 97.0-102.6% in hydrochloric acid, 99.5-100.2% in acetate buffer, 98.3–100.1% in phthalate buffer. These methods were found to be simple, accurate, reproducible and economical and can be used for the routine analysis of gatifloxacin in bulk drug and its tablets.