Development and Validation of Spectrophotometric Method for Simultaneous Estimation of Tramadol Hydrochloride and Paracetamol from their Combined Dosage Form

A Simple, accurate and precise method for simultaneous estimation of Tramadol Hydrochloride and Paracetamol in their combined dosage form have been described. The spectrophotometric method is based on zero absorbance measurement, which uses 272 and 250 nm as two analytical wavelengths. Beer’s law was obeyed in the concentration range of 60 to 160 μg/ml for Tramadol hydrochloride and 8 to 30 μg/ml for Paracetamol. The LOD and LOQ were found to be 25 μg/ml and 60 μg/ml for Tramadol hydrochloride and 3 μg/ml and 8 μg/ml for Paracetamol, respectively. The results obtained in the analysis of dosage form by developed method are well agreed with the labeled contents.