Simultaneous Determination of Valsartan and Nebivolol HCl in Tablet Dosage Form by RP-HPLC

A simple, fast and precise reversed phase high performance liquid chromatographic method is developed for the simultaneous determination of valsartan and nebivolol in tablet dosage form. Chromatographic separation of these drugs was performed on Kromasil C₁₈ column (250×4.6 mm, 5μ) as stationary phase with a mobile phase comprising of 20 mM potassium dihydrogen orthophosphate: acetonitrile in the ratio of 43:57 (v/v) containing 0.1% glacial acetic acid at a flow rate of 1 ml/min and UV detection at 282 nm. The linearity of valsartan and nebivolol were in the range of 40 to 96 μg/mL and 2.5 to 6.0 μg/mL respectively. The recovery was calculated by standard addition method. The average recoveries were found to be 99.32% and 99.38% for valsartan and nebivolol respectively. The proposed method was found to be accurate, precise and rapid for simultaneous determination of valsartan and nebivolol.