Asian Journal of Research in Chemistry

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Purification and Characterization of GroES and GroEL from Genetically Modified Strain E. coli U1/Pus01/Puss1ΔCat


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1 Department of Molecular Biology, IMS BHU, Varanasi-221005, India
     

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In Present study our aim is to purify and characterize GroES and GroEL from genetically modified strain E.coli U1/pUS01/pUSS1ΔCAT L. GroES was eluted at 0.2 M NaCl and GroEL was at 0.3 M NaCl. From 1.4 litres culture 7.5 mg GroEL and 50 mg GroES was purified.

Keywords

Cheperonin, GroEL, GroES, IPTG, Q-Sepharose FF, Sephacryl S-200 HR.
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  • Purification and Characterization of GroES and GroEL from Genetically Modified Strain E. coli U1/Pus01/Puss1ΔCat

Abstract Views: 155  |  PDF Views: 0

Authors

Alok Kumar Verma
Department of Molecular Biology, IMS BHU, Varanasi-221005, India
G. P. Pal
Department of Molecular Biology, IMS BHU, Varanasi-221005, India

Abstract


In Present study our aim is to purify and characterize GroES and GroEL from genetically modified strain E.coli U1/pUS01/pUSS1ΔCAT L. GroES was eluted at 0.2 M NaCl and GroEL was at 0.3 M NaCl. From 1.4 litres culture 7.5 mg GroEL and 50 mg GroES was purified.

Keywords


Cheperonin, GroEL, GroES, IPTG, Q-Sepharose FF, Sephacryl S-200 HR.