Asian Journal of Research in Chemistry

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Development and Validation of HPTLC Method for Simultaneous Estimation of Omeprazole and Ondansetron in Tablet Dosage Form


Affiliations
1 Department of Pharmaceutical Analysis, Veerayatan Institute of Pharmacy, Kutch, India
2 Ramanbhai Patel College of Pharmacy, Changa, Gujarat, India
3 Veeda, Ahmedabad, Gujarat, India
4 A. R. College of Pharmacy, Vallabh, Vidhyanagar, India
     

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A simple, precise, sensitive, rapid and reproducible HPTLC method for the simultaneous estimation of the omeprazole and ondansetron in tablets was developed and validated. This method involves separation of the components by HPTLC on precoated silica gel G60F254 plate with solvent system of methanol: ethyl acetate: toluene(2:6:2% v/v) and detection was carried out densitometrically using a UV detector at 280 nm in absorbance mode. This system was found to give compact spots for omeprazole (Rf value of 0.68±0.02) and for ondansetron (Rf value of 0.17±0.02). Linearity was found to be in the range of 1000-3500 ng/spot and 1000-3500 ng/spot for omeprazole and ondansetron respectively. The limit of detection and limit of quantification for omeprazole were 123.42 and 374.01 ng/spot and for ondansetron were 134.63 and 407.97 ng/spot, respectively. The method was found to be beneficial for the routine analysis of combined dosage form.

Keywords

Simultaneous Estimation, HPTLC, Omeprazole, Ondansetron.
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  • Development and Validation of HPTLC Method for Simultaneous Estimation of Omeprazole and Ondansetron in Tablet Dosage Form

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Authors

Zarna Dedania
Department of Pharmaceutical Analysis, Veerayatan Institute of Pharmacy, Kutch, India
Ronak Dedania
Department of Pharmaceutical Analysis, Veerayatan Institute of Pharmacy, Kutch, India
G. Vidhyasagar
Department of Pharmaceutical Analysis, Veerayatan Institute of Pharmacy, Kutch, India
Bhavna Patel
Ramanbhai Patel College of Pharmacy, Changa, Gujarat, India
Chetan Ramolia
Veeda, Ahmedabad, Gujarat, India
Vaishali Karkhanis
A. R. College of Pharmacy, Vallabh, Vidhyanagar, India

Abstract


A simple, precise, sensitive, rapid and reproducible HPTLC method for the simultaneous estimation of the omeprazole and ondansetron in tablets was developed and validated. This method involves separation of the components by HPTLC on precoated silica gel G60F254 plate with solvent system of methanol: ethyl acetate: toluene(2:6:2% v/v) and detection was carried out densitometrically using a UV detector at 280 nm in absorbance mode. This system was found to give compact spots for omeprazole (Rf value of 0.68±0.02) and for ondansetron (Rf value of 0.17±0.02). Linearity was found to be in the range of 1000-3500 ng/spot and 1000-3500 ng/spot for omeprazole and ondansetron respectively. The limit of detection and limit of quantification for omeprazole were 123.42 and 374.01 ng/spot and for ondansetron were 134.63 and 407.97 ng/spot, respectively. The method was found to be beneficial for the routine analysis of combined dosage form.

Keywords


Simultaneous Estimation, HPTLC, Omeprazole, Ondansetron.