Asian Journal of Research in Chemistry

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Development and Validation of RP-HPLC Method for Simultaneous Determination of Ceftriaxone Sodium and Sulbactam Sodium in Bulk and Pharmaceutical Formulation


Affiliations
1 School of Pharmacy Technology and Management, SVKM’s, NMIMS University, Shirpur Campus, Babulde, Near Bank of Tapi River, Shirpur-425405, Dist Dhule (M.S), India
2 Research and Development Division, Concept Pharmaceuticals Ltd, Aurangabad, India
     

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A reverse phase high performance liquid chromatographic method was developed for simultaneous determination of Ceftriaxone Sodium and Sulbactam Sodium in bulk and Pharmaceutical Formulation. The separation was done by a Kromasil C18 column (250 mm×4.6 mm, 5 μm) using Acetonitrile: Buffer PH 7: Distilled Water (400:50:550, v/v/v) as mobile phase. The validation of the method was performed, and specificity, reproducibility, precision and accuracy were confirmed. The limit of detection was approximately 0.39 μg/ml for Ceftriaxone Sodium and 0.08 μg/ml for Sulbactam sodium. Due to simplicity and accuracy the method particularly suitable for routine pharmaceutical quality control.

Keywords

RP-HPLC, Ceftriaxone Sodium, Sulbactam Sodium.
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  • Development and Validation of RP-HPLC Method for Simultaneous Determination of Ceftriaxone Sodium and Sulbactam Sodium in Bulk and Pharmaceutical Formulation

Abstract Views: 246  |  PDF Views: 0

Authors

Jagjitsingh C. Girase
School of Pharmacy Technology and Management, SVKM’s, NMIMS University, Shirpur Campus, Babulde, Near Bank of Tapi River, Shirpur-425405, Dist Dhule (M.S), India
G. Venkateshwarlu
Research and Development Division, Concept Pharmaceuticals Ltd, Aurangabad, India
Saurabh Kumar Banerjee
School of Pharmacy Technology and Management, SVKM’s, NMIMS University, Shirpur Campus, Babulde, Near Bank of Tapi River, Shirpur-425405, Dist Dhule (M.S), India

Abstract


A reverse phase high performance liquid chromatographic method was developed for simultaneous determination of Ceftriaxone Sodium and Sulbactam Sodium in bulk and Pharmaceutical Formulation. The separation was done by a Kromasil C18 column (250 mm×4.6 mm, 5 μm) using Acetonitrile: Buffer PH 7: Distilled Water (400:50:550, v/v/v) as mobile phase. The validation of the method was performed, and specificity, reproducibility, precision and accuracy were confirmed. The limit of detection was approximately 0.39 μg/ml for Ceftriaxone Sodium and 0.08 μg/ml for Sulbactam sodium. Due to simplicity and accuracy the method particularly suitable for routine pharmaceutical quality control.

Keywords


RP-HPLC, Ceftriaxone Sodium, Sulbactam Sodium.