Simultaneous Spectrophotometric Estimation of Levofloxacin Hemihydrate and Ornidazole in Pharmaceutical Tablet Dosage Form

Two sensitive, simple, Rapid, accurate and precise methods for simultaneous estimation of Levofloxacin hemihydrate (LEVO) and Ornidazole (ONZ) in two component solid dosage forms have been developed. The methods employ the application of simultaneous equation and the absorbance ratio (Q-analysis). All these methods utilize 0.1 M HCl as a solvent. LEVO shows maximum absorbance at a wavelength of 293 nm and ONZ at 277 nm. where the linearity ranges for LEVO and ONZ were 2-14 μg/ml and 4-36 μg/ml. respectively. Determination of ratio of absorbance at 293 nm. The maximum absorption of LEVO and isobestic wavelength 270 nm. the linearity ranges for LEVO and ONZ were 2-20 μg/ml and 4-40 μg/ml. respectively. The procedures were successfully applied for the simultaneous determination of both drugs in laboratory prepared mixtures and in commercial tablet preparation. The accuracy of the methods was assessed by recovery studies and was found to be 99.73%±0.0050 and 99.09%±0.0191 for LEVO and ONZ respectively by the simultaneous equation method. and 99.72%±0.0166 and 99.67%±0.00626 for LEVO and ONZ respectively the graphical absorbance ratio method.