Comparative Estimation of Curcumin Content from Marketed Herbal Anti Rheumatic Tablets Formulation

A rapid and sensitive high-performance thin-layer chromatographic (HPLC) method was developed and validated for the quantitative estimation of Curcumin in formulation containing Curcuma longa extracts. Simple extraction method was used for isolation of curcumin from formulation sample. The isolated samples were chromatographed by using RP-HPLC Method on HiQ Sil C18 W 4.6 mm×250 mm i.d. column and detected by UV at 420 nm. The linearity range for Curcumin was 0.4-2.4 mcg/ml with average recovery of 97.57±0.5137. The limit of detection and limit of quantification was found to be 3.0196 and 9.1503 for curcumin respectively.The developed method was successfully applied for the assay of market formulations containing Curcuma longa extracts.