RP-HPLC Determination of Levetiracetam in Bulk and Pharmaceutical Formulation

An isocratic reversed phase liquid chromatographic (RP-HPLC) method has been developed and subsequently validated for the determination of Levetiracetam in bulk and pharmaceutical formulation. Separation was achieved with a Hypersil Gold 150×4.6 mm; 5μ column with Mixture of Potassium Dihydrogen Phosphate and Sodium 1-Heptane Sulphonate (pH adjusted to 2.8±0.05 with Orthophosphoric acid):Acetonitrile (90:10, v/v) as eluent at a flow rate 1.0 ml/min. UV detection was performed at 220 nm. The method is simple, rapid, selective and stability indicating. The described method is linear over a range of 43.444 μg/mL to 289.629 μg/mL. The method precision for the determination of assay was below 1.0% RSD. The Percentage recoveries of Active Pharmaceutical Ingredient(API) from dosage forms ranged from 100.2 to 101.0. The method is useful in the quality control of bulk manufacturing and also in pharmaceutical formulations.