Asian Journal of Research in Chemistry

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Simultaneous RP-HPLC Estimation of Nitazoxanide and Ofloxacin in Tablet Dosage Forms


Affiliations
1 Dept. of Pharmaceutical Analysis, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, Tamilnadu, India
     

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Reverse Phase HPLC method for the determination of nitazoxanide and ofloxacin in bulk and tablet formulations. The determination was carried out by using Phenomenex C18 column with 0.24% sodium lauryl sulphate:acetonitrile:acetic acid (pH-4.0) (58:40:02) as the mobile phase. The flow rate was 1.5 ml/min and the eluent was monitored at 295 nm. The Retention time of nitazoxanide and ofloxacin were 2.2 and 5.4 respectively. Linearity for the nitazoxanide and ofloxacin were found in the range of 400-600 μg/ml and 160-240 μg/ml respectively. The method was reproducible, with good resolution between nitazoxanide and ofloxacin and can be use for routine analysis.

Keywords

RP-HPLC, Nitazoxanide and Ofloxacin.
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  • Simultaneous RP-HPLC Estimation of Nitazoxanide and Ofloxacin in Tablet Dosage Forms

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Authors

R. Siva Kumar
Dept. of Pharmaceutical Analysis, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, Tamilnadu, India
P. Kumar Nallasivan
Dept. of Pharmaceutical Analysis, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, Tamilnadu, India
S. Saravanakumar
Dept. of Pharmaceutical Analysis, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, Tamilnadu, India
C. S. Kandasamy
Dept. of Pharmaceutical Analysis, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, Tamilnadu, India
R. Venkatnarayanan
Dept. of Pharmaceutical Analysis, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, Tamilnadu, India

Abstract


Reverse Phase HPLC method for the determination of nitazoxanide and ofloxacin in bulk and tablet formulations. The determination was carried out by using Phenomenex C18 column with 0.24% sodium lauryl sulphate:acetonitrile:acetic acid (pH-4.0) (58:40:02) as the mobile phase. The flow rate was 1.5 ml/min and the eluent was monitored at 295 nm. The Retention time of nitazoxanide and ofloxacin were 2.2 and 5.4 respectively. Linearity for the nitazoxanide and ofloxacin were found in the range of 400-600 μg/ml and 160-240 μg/ml respectively. The method was reproducible, with good resolution between nitazoxanide and ofloxacin and can be use for routine analysis.

Keywords


RP-HPLC, Nitazoxanide and Ofloxacin.