Asian Journal of Research in Chemistry

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Chlorpyrifos biodegradation by a Locally Isolated Bacterium Pseudomonas sp. B5-2


Affiliations
1 Laboratory of Molecular Toxicology, Faculty of Nature and Life Sciences, University of Mohammed Seddik Benyahia-Jijel, Jijel, Algeria ., India
2 Laboratory of Molecular Toxicology, Faculty of Nature and Life Sciences, University of Mohammed Seddik Benyahia-Jijel, Jijel, Algeria., India
3 Department of Applied Microbiology and Food Sciences, Faculty of Nature and Life Sciences, University Mohammed Seddik Benyahia-Jijel, Jijel, Algeria.
4 Department of Applied Microbiology and Food Sciences, Faculty of Nature and Life Sciences, University Mohammed Seddik Benyahia-Jijel, Jijel, Algeria., India
     

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Chlorpyrifos has been considered as one of the most frequently used organophosphorus insecticides in agriculture worldwide. There has been considerable public concern regarding its neurotoxicity and its genotoxicity on mammals, aquatic organisms, and other non-target species. In this study 23 bacterial isolated strains from different origins were tested for their ability to resist and subsequently degrade chlorpyrifos (CP). Seven of these strains showed a resistance to up to 1000mg/L CP on solid minimal mineral salt medium (MMSM) and the strain Pseudomonas sp. B5-2 was selected for further analysis after a selection on liquid MMSM. The effect of different concentrations of CP on the growth of the selected strain was tested, and 200 mg/L was the highest concentration that supports its growth. The ability of the selected strain to eliminate CP was investigated in the presence and in the absence of glucose as an external carbon source. The results showed that the strain utilized chlorpyrifos (200 mg/L) as the sole source of carbon better than in presence of an external carbon source (glucose) with an elimination rate of 67.06% and 14.61%, respectively. These results highlight the potential of this bacterium to be used in the cleanup of contaminated pesticide waste in the environment.

Keywords

Biodegradation, Chlorpyrifos, Co-metabolism, Pesticide, Pseudomonas sp. B5-2.
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  • Aloui N. Ouled-Haddar H. Bissati S.K. Omeiri N. Pesticide occurrence and distribution in cultivated soils of the arid region of el-hejera, ouargla province and correlation with soil properties.Journal of Fundamental and Applied Sciences. 2020; 12(1): 35-48.doi: http://dx.doi.org/10.4314/jfas.v12i1.4

  • Virgilio R. Góngora E. Rodrigo G. Rojas-Herrera R. GiácomanVallejos G. Ponce-Caballero C. Pesticide bioremediation in liquid media using a microbial consortium and bacteria-pure strains isolated from a biomixture used in agricultural areas. Ecotoxicology and Environmental Safety. 2020; 200:110734.

  • https://doi.org/10.1016/j.ecoenv.2020.110734.

  • Supta D. Kimberly J. Madeleine T. Sue M. Ian S. Fate of the organophosphate insecticide, chlorpyrifos, in leaves, soil, and air following application. Chemosphere. 2020; 243: 125194. https://doi.org/10.1016/j.chemosphere.2019.125194.

  • Abraham J. Silambarasan S. Biodegradation of chlorpyrifos and its hydrolysis product 3,5,6-trichloro-2-pyridinol using a novel bacterium Ochrobactrum sp. JAS2: A proposal of its metabolic pathway. Pesticide Biochemistry and Physiology. 2026; 126: 1321. https://doi.org/10.1016/j.pestbp.2015.07.001.

  • Duraisamy K. Muthusamy S. Balakrishnan S. An eco-friendly detoxification of chlorpyrifos by Bacillus cereus MCAS02 native isolate from agricultural soil, Namakkal, Tamil Nadu, India, Biocatalysis and Agricultural Biotechnology. 2018; 13: 283-290.https://doi.org/10.1016/j.bcab.2018.01.001.

  • Ahirwar U. Kollah B. Dubey G. Mohanty S. Chlorpyrifos biodegradation in relation to metabolic attributes and 16S rRNA gene phylogeny of bacteria in a tropical vertisol. SN Applied Sciences. 2019; 1: 228. https://doi.org/10.1007/s42452-019-0206-0

  • Shi T. Fang L. Qin H. Chen Y., Wu X. Hua R. Rapid Biodegradation of the Organophosphorus Insecticide Chlorpyrifos by Cupriavidus nantongensis X1T. International Journal of Environmental Research and Public Health. 2019; 16(23): 4593.doi :10.3390/ijerph16234593

  • Singh B K. Walker A. Morgan J A. Wright D J. Biodegradation of chlorpyrifos by Enterobacter strain B-14 and its use in bioremediation of contaminated soils. Applied Environmental Microbiology. 2014; 70(8): 4855-4863. doi: 10.1128/AEM.70.8.4855-4863.2004

  • Yang L. Zhao Y. Zhang B. Yang C. Zhang X. Isolation and characterization of a chlorpyrifos and 3,5,6-trichloro-2-pyridinol degrading bacterium. FEMS Microbiology Letters. 2005; 251(1): 67–73. https://doi.org/10.1016/j.femsle. 2005.07.031

  • Rayu S. Nielsen U N. Nazaries L. Singh B K. Isolation and Molecular Characterization of Novel Chlorpyrifos and 3,5,6trichloro-2-pyridinol-degrading Bacteria from Sugarcane Farm Soils. Frontiers in Microbiology. 2017;8: 518. doi: 10.3389/fmicb.2017.00518

  • Cycon M. Wójcik M. Piotrowska-Seget, Z. Biodegradation of the Organophosphorus Insecticide Diazinon by Serratia Sp. and Pseudomonas Sp. and Their Use in Bioremediation of Contaminated Soil. Chemosphere. 2009; 76 :494–501.

  • Anwar S. Liaquat F. Khan Q M. Khalid Z M. Iqbal S. Biodegradation of Chlorpyrifos and Its Hydrolysis Product 3,5,6Trichloro-2-Pyridinol by Bacillus Pumilus Strain C2A1. Journal of Hazardous Materials. 2009; 168: 400–405.

  • Youness M. Impact de La Formulation et du Mélange de deux Pesticides (mésotrione et tébuconazole) sur leur Biodégradation et La Croissance de Microorganismes. 2014. Université Blaise Pascal- Clermont-Ferrand, France

  • Supreeth M. Chandrashekar M. Sachin N. Raju N. Effect of chlorpyrifos on soil microbial diversity and its biotransformation by Streptomyces sp. HP-11. 3. Biotechnology. 2016; 6: 147. https://doi.org/10.1007/s13205-016-0462-2

  • Hossain M. Chowdhury M. Pramanik M K. Rahman M A. Fakhruddin A N. Alam M K. Determination of selected pesticides in water samples adjacent to agricultural fields and removal of organophosphorus insecticide chlorpyrifos using soil bacterial isolates. Applied Water Science. 2015; 5: 171–179.

  • https://doi.org/10.1007/s13201-014-0178-6

  • Aissaoui S. Ouled-Haddar H. Sifour M. Harrouche K. Sghaier H. Metabolic and Co-Metabolic Transformation of Diclofenac by Enterobacter hormaechei D15 Isolated from Activated Sludge.

  • Current Microbiology. 2017; 74: 381–388.https://doi.org/10.1007/s00284-016-1190-x

  • Rani M S. Lakshmi K V. Devi P S. Madhuri R J. Aruna S. Jyothi K. Narasimha G. Venkateswarlu K. Isolation and Characterization of a Chlorpyrifos-Degrading Bacterium from Agricultural Soil and its Growth Response. African Journal of Microbiology. Res.2008; 2:26-31.

  • Verma J. Jaiswal D. Maurya P. Screening of bacterial strains for developing effective pesticide-tolerant plant growth-promoting microbial consortia from rhizosphere soils of vegetable fields of eastern Uttar Pradesh, India. Energy, Ecology and Environment. 2016;1: 408–418. https://doi.org/10.1007/s40974-016-0028-5

  • Lakshmi C. Kumar M. Khanna S. Biotransformation of Chlorpyrifos and Bioremediation of Contaminated. Soil. Int. Biodeterioration Biodegradation. 2008; 62: 204–209. doi: 10.1016/j.ibiod.2007.12.005

  • Latifi A. Khodi S. Mirzaei M. Miresmaeili M. Babavalian H. Isolation and Characterization of Five Chlorpyrifos degrading Bacteria. African Journal of Biotechnology. 2012; 11(13): 31403146. doi: 10.5897/AJB11.2814

  • Kumar S. Kaushik G. Dar M. Nimesh S. L´opez-Chuken U J. Villarreal-Chiu J. Microbial degradation of organophosphate pesticides: A review. Pedosphere. 2018; 28(2): 1–201. doi:10.1016/S1002-0160(18)60017-7

  • Singh B. Walker A. Microbial degradation of organophosphorus compounds, FEMS Microbiology Reviews. 2006; 30(3): 428–471. https://doi.org/10.1111/j.1574-6976.2006.00018.x

  • Groning J. Held C. Garten C. Claubnitzer U. Kaschabek SR. Schlomann M. Transformation of diclofenac by the indigenous microflora of river sediments and identification of a major intermediate. Chemosphere. 2007; 69: 509-516. doi: 10.1016/j.chemosphere.2007.03.037

  • Sharma N. Lavania M. Lal B. Microbes and their Secondary Metabolites: Agents in Bioremediation of Hydrocar-bon Contaminated Site. Arch Pet Environ Biotechnol. 2019; 4: 151. doi: 10.29011/2574-7614.100051

  • Cerqueira V. Hollenbach E. Maboni F. Vainstein M. Camargo F. Do Carmo R. Peralba M. Bento F M. Biodegradation potential of oily sludge by pure and mixed bacterial cultures. Bioresource Technology. 2011; 102(23): 11003-10. doi: 10.1016/j.biortech. 2011. 09.074 .


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  • Chlorpyrifos biodegradation by a Locally Isolated Bacterium Pseudomonas sp. B5-2

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Authors

Aissaoui S
Laboratory of Molecular Toxicology, Faculty of Nature and Life Sciences, University of Mohammed Seddik Benyahia-Jijel, Jijel, Algeria ., India
Sifour M
Laboratory of Molecular Toxicology, Faculty of Nature and Life Sciences, University of Mohammed Seddik Benyahia-Jijel, Jijel, Algeria., India
Sifour M
Department of Applied Microbiology and Food Sciences, Faculty of Nature and Life Sciences, University Mohammed Seddik Benyahia-Jijel, Jijel, Algeria.
Abdelli M
Department of Applied Microbiology and Food Sciences, Faculty of Nature and Life Sciences, University Mohammed Seddik Benyahia-Jijel, Jijel, Algeria., India
Meribai N
Department of Applied Microbiology and Food Sciences, Faculty of Nature and Life Sciences, University Mohammed Seddik Benyahia-Jijel, Jijel, Algeria., India
Ouled- Haddar H
Laboratory of Molecular Toxicology, Faculty of Nature and Life Sciences, University of Mohammed Seddik Benyahia-Jijel, Jijel, Algeria., India
Ouled- Haddar H
Department of Applied Microbiology and Food Sciences, Faculty of Nature and Life Sciences, University Mohammed Seddik Benyahia-Jijel, Jijel, Algeria., India

Abstract


Chlorpyrifos has been considered as one of the most frequently used organophosphorus insecticides in agriculture worldwide. There has been considerable public concern regarding its neurotoxicity and its genotoxicity on mammals, aquatic organisms, and other non-target species. In this study 23 bacterial isolated strains from different origins were tested for their ability to resist and subsequently degrade chlorpyrifos (CP). Seven of these strains showed a resistance to up to 1000mg/L CP on solid minimal mineral salt medium (MMSM) and the strain Pseudomonas sp. B5-2 was selected for further analysis after a selection on liquid MMSM. The effect of different concentrations of CP on the growth of the selected strain was tested, and 200 mg/L was the highest concentration that supports its growth. The ability of the selected strain to eliminate CP was investigated in the presence and in the absence of glucose as an external carbon source. The results showed that the strain utilized chlorpyrifos (200 mg/L) as the sole source of carbon better than in presence of an external carbon source (glucose) with an elimination rate of 67.06% and 14.61%, respectively. These results highlight the potential of this bacterium to be used in the cleanup of contaminated pesticide waste in the environment.

Keywords


Biodegradation, Chlorpyrifos, Co-metabolism, Pesticide, Pseudomonas sp. B5-2.

References