Asian Journal of Research in Chemistry

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Development of fingerprinting method for Siddha formulation Nilaavaarai chooranam: A HPTLC approach


Affiliations
1 Chhattisgarh Food and Drug Administration, Mahasamund (CG)., India
2 University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur (C.G.)., India
3 Institute of Pharmacy, Vikram University, Ujjain (MP)., India
     

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Selective and efficient analytical methods are required not only for quality assurance but also for authentication of herbal formulations. Fingerprint method for Nilaavaarai Chooranam with TLC Densitometric Methods (HPTLC) using piperine as an internal standard was developed. The HPTLC estimation was carried out with three laboratory batches and one marketed formulation of Nilaavaarai Chooranam and crude drug containing piperine. The concentration of piperine present in raw material was found to be 2.9836±0.497w/w in Piper nigrum and in three identical laboratory batch of Nilaavaarai Chooranam NC-I, NC-II and N-III, was found to be 0.1761 ± 0.384, 0.1759 ± 0.534%, 0.1760 ± 0.019 w/w respectively. The piperine content in all the three different batches is found to be in close proximities with each other. The results were comparable to marketed formulations. Hence the present method is simple, sensitive, precise and accurate and can be adopted for routine fingerprinting method for quality control of Nilaavaarai Chooranam.

Keywords

Nilaavaarai chooranam, Piperine, Sidhha formulation, HPTLC, Standardization.
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  • Jain V., Saraf S. and Saraf S. Spectrophotometric determination of Piperine in Trikatu Churna: An Ayurvedic Formulation, Asian Journal of Chemistry. 2007, 19, (7), 5331-5335.

  • World Health Organization, Quality Control Methods for Medicinal Plants Materials, Geneva, 1998, 1-15.

  • WHO general guidelines for methodologies on research and evaluation of traditional medicine. (2000): http://whqlibdoc.who.int/hq/2000/WHO_EDM_TRM_2000.1.pdf.

  • Gupta M., Chaudhary P.H., Mukund G. Shrivastava T.B., Need and scope of standardization of herbal medicines - A review, International Journal of Green Pharmacy. Oct-Dec 2021 ,16 (1), 346-352.

  • Formulary of Siddha Medicine. 4th ed., Published by Indian Medical Practitioners Cooperative Pharmacy and Store Ltd (IMPCOPS), Chennai, 1993: 45.

  • Indian Herbal Pharmacopoeia, Regional Research Laboratory Jammu, Indian drug Manufacturing Association Mumbai. 1999.

  • Gurinderdeep S, Piperine: A Remarkable Marker with Intense Biological Activity, International Journal of Pharmacognosy and Chinese Medicine. 2017, 1(4): 122.


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  • Development of fingerprinting method for Siddha formulation Nilaavaarai chooranam: A HPTLC approach

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Authors

Tripti Jain
Chhattisgarh Food and Drug Administration, Mahasamund (CG)., India
Amber Vyas
University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur (C.G.)., India
Darshan Dubey
Institute of Pharmacy, Vikram University, Ujjain (MP)., India
Kamlesh Dashora
Institute of Pharmacy, Vikram University, Ujjain (MP)., India
Vishal Jain
University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur (C.G.)., India

Abstract


Selective and efficient analytical methods are required not only for quality assurance but also for authentication of herbal formulations. Fingerprint method for Nilaavaarai Chooranam with TLC Densitometric Methods (HPTLC) using piperine as an internal standard was developed. The HPTLC estimation was carried out with three laboratory batches and one marketed formulation of Nilaavaarai Chooranam and crude drug containing piperine. The concentration of piperine present in raw material was found to be 2.9836±0.497w/w in Piper nigrum and in three identical laboratory batch of Nilaavaarai Chooranam NC-I, NC-II and N-III, was found to be 0.1761 ± 0.384, 0.1759 ± 0.534%, 0.1760 ± 0.019 w/w respectively. The piperine content in all the three different batches is found to be in close proximities with each other. The results were comparable to marketed formulations. Hence the present method is simple, sensitive, precise and accurate and can be adopted for routine fingerprinting method for quality control of Nilaavaarai Chooranam.

Keywords


Nilaavaarai chooranam, Piperine, Sidhha formulation, HPTLC, Standardization.

References