Asian Journal of Research in Chemistry

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The impact of pH value in the Phenolic content and Antioxidant potential of Medicinal plant extract


Affiliations
1 Department of Process Engineering, Faculty of Technology, University of Echahid Hamma Lakhdar, El Oued 39000, Algeria ., India
2 Unit of Renewable Energy Development in Arid Zones (UDERZA), El Oued 39000, Algeria ., India
3 Department of Process Engineering, Faculty of Technology, University of Echahid Hamma Lakhdar, El Oued 39000, Algeria., India
4 Unit of Renewable Energy Development in Arid Zones (UDERZA), El Oued 39000, Algeria.
5 Laboratoire De Biotechnologie Biomatériaux et Matériaux Condensés, Faculty of Technology, University Echahid Hammam Lakhdar El Oued Algeria ., India
     

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In this research, the aim was to investigate the influence of pH value in the extraction medium on the phytochemical composition and antioxidant activity of Artemisia herba-alba species used in traditional medicine. For this reason, two analytical methods were used (UV-visible spectroscopy, HPLC). Antioxidant properties of the extracts at different pH were estimated by two tests: total antioxidant capacity (TAC) and DPPH free radical scavenging capacity. The results of the evaluation of phenolic compounds showed that the aqueous extracts in a weak acid medium had a high percentage of phenol content, and the highest value for total phenol content was 90.759±1.267mg GAE/g. Whereas, the HPLC analysis indicated the presence of most of the standard phenolic compounds in the majority of the extracts. For antioxidant activity, the best antioxidants reported in extract at pH=6, with a semi-maximal inhibitory concentration (0.151±0,032mg/ml) and a total antioxidant capacity (TAC) of 66.840±0.241mg EAA/g, which indicates that it is effective antioxidant for therapeutic applications .

Keywords

Artemisia herba-alba, pH, polyphenols, HPLC, antioxidant activity.
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  • The impact of pH value in the Phenolic content and Antioxidant potential of Medicinal plant extract

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Authors

Berra Djamila
Department of Process Engineering, Faculty of Technology, University of Echahid Hamma Lakhdar, El Oued 39000, Algeria ., India
Berra Djamila
Unit of Renewable Energy Development in Arid Zones (UDERZA), El Oued 39000, Algeria ., India
Dia Ouahida
Department of Process Engineering, Faculty of Technology, University of Echahid Hamma Lakhdar, El Oued 39000, Algeria., India
Dia Ouahida
Unit of Renewable Energy Development in Arid Zones (UDERZA), El Oued 39000, Algeria.
Laouini Salah Eddine
Department of Process Engineering, Faculty of Technology, University of Echahid Hamma Lakhdar, El Oued 39000, Algeria., India
Laouini Salah Eddine
Laboratoire De Biotechnologie Biomatériaux et Matériaux Condensés, Faculty of Technology, University Echahid Hammam Lakhdar El Oued Algeria ., India
Menaceur Souheila
Department of Process Engineering, Faculty of Technology, University of Echahid Hamma Lakhdar, El Oued 39000, Algeria ., India
Menaceur Souheila
Laboratoire De Biotechnologie Biomatériaux et Matériaux Condensés, Faculty of Technology, University Echahid Hammam Lakhdar El Oued Algeria ., India

Abstract


In this research, the aim was to investigate the influence of pH value in the extraction medium on the phytochemical composition and antioxidant activity of Artemisia herba-alba species used in traditional medicine. For this reason, two analytical methods were used (UV-visible spectroscopy, HPLC). Antioxidant properties of the extracts at different pH were estimated by two tests: total antioxidant capacity (TAC) and DPPH free radical scavenging capacity. The results of the evaluation of phenolic compounds showed that the aqueous extracts in a weak acid medium had a high percentage of phenol content, and the highest value for total phenol content was 90.759±1.267mg GAE/g. Whereas, the HPLC analysis indicated the presence of most of the standard phenolic compounds in the majority of the extracts. For antioxidant activity, the best antioxidants reported in extract at pH=6, with a semi-maximal inhibitory concentration (0.151±0,032mg/ml) and a total antioxidant capacity (TAC) of 66.840±0.241mg EAA/g, which indicates that it is effective antioxidant for therapeutic applications .

Keywords


Artemisia herba-alba, pH, polyphenols, HPLC, antioxidant activity.

References