Asian Journal of Research in Chemistry

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Evaluation of the Antilithiasic activity, Phospho-ammoniaco-magnésien of the aqueous extract of Fraxinus excelsior (L.)


Affiliations
1 Laboratory of Biochemistry and Applied Microbiology, Badji Mokhtar Annaba University, 23000, Algeria., India
2 Laboratory of Biophysics, Faculty of Medicine, Annaba, 23000, Algeria., India
3 Research Unit Materials Science and Applications, Constantine University, 25000, Algeria., India
4 Institute of Agronomic and Veterinary Sciences, Departement of Veterinary Medicine, University of Souk Ahras, 41000, Algeria., India
5 Institute of Agronomic and Veterinary Sciences, Departement of Veterinary Medicine, University of Souk Ahras, 41000, Algeria ., India
6 Environmental Research Center (ERC), Annaba, 23000, Algeria ., India
     

   Subscribe/Renew Journal


The formation of urinary stones is a serious clinical problem in the world, the struvite of which is one of the characteristic components of urolithiasis of infectious origin. The objective of this study is to evaluate the in vitro litholytic and inhibitory effects of the leaf aqueous extract of Fraxinus excelsior L plant on the crystallization of struvite. The plant is commonly used in traditional Algerian medicine.A characterization method of Fraxinus excelsior L plant was first performed to determine the total phenolic and flavonoid compounds of Fraxinus excelsior L leaf aqueous extract. Struvite crystals prepared with two different processes had subsequently undergone an inhibition assay by supplementing Aqueous Extract with varying concentrations. In the first process, struvite was biologically crystallized in a synthetic urine in the presence of Proteus mirabilis during 24 hours of incubation period. This was followed by the addition of Aqueous Extract, the effect of which was evaluated after 1-hour. In the second process, struvite crystals were chemically synthesized for 30 minutes in the presence of aqueous extract. These two processes allow to assess, respectively, the litholytic effect of aqueous extract on the previously formed struvite crystals and the inhibitory effect of the crystallization of struvite. These various tests of crystallization and inhibition of struvite crystals were conducted by Polarized Light Microscopy (PLM) and Fourier Transform Infrared (FTIR) Spectroscopy. The results reveal that this Aqueous Extract contains an estimated total phenolic content of 60.92 ± 0.94 mg gallic acid equivalent (GAE)/g of extract and a total flavonoid content of 19.32 ± 0.75 mg quercetin equivalent (QE)/g of extract. The in vitro struvite crystals dissolution testing revealed a significant litholytic effect. Also, the struvite crystallization inhibition testing showed that it exerted a significant inhibitory effect observed with polarized light.The effect of this extract is evident against struvite crystals with a conclusive litholytic and inhibitory potential. The present results open up significant preventive management prospects for this type of lithiasis.

Keywords

Fraxinus excelsior, Urinary Lithiasis, Struvite; Litholytic Effect, Crystal Inhibition
Subscription Login to verify subscription
User
Notifications
Font Size


  • Khitri W, Lachgueur N, Tasfaout A, Lardjam A et Khalfa A. Plantes antilithiasiques utilisées en médecine traditionnelle dans la ville d’Oran, Algérie: Approche ethnobotanique et phytochimique. Revue d’ethnoécologie 2016; 9: 2267–2419. https://doi.org/10.4000/ethnoecologie.2511

  • Neeraja Kamakshi U, Ganga Rao B, Venkateswara Rao B. Comparison of In vitro Antiurolithiatic Activity of Aervalanata, Sphaeranthusindicus, Merremiaemarginata. Research J. Pharm. and Tech. 2017; 10(6): 1653-1656. doi: 10.5958/0974-360X.2017.00291.8

  • Amritan Kachru, Monika Bisht, Mamta Baunthiyal. In vitro evaluation of anti-neprolithiatic activity of leaves and seeds of Macrotylomauniflorum on dissolution or removal of kidney stones. Res. J. Pharmacognosy& Phytochem.2016; 8(1): 05-12. doi: 10.5958/0975-4385.2016.00002.9

  • Dileep Singh Baghel, Amit Mittal, Saurabh Singh, Anand Kumar Chaudhary, Amit Bhatia, Shruti Chopra. Formulation, Evaluation and Assessment of In Vitro Potential of Gokshur Ghan Tablet against Urolithiasis (Mutrakrichra). Research Journal of Pharmacy and Technology. 2021; 14(4):1945-2. doi: 10.52711/0974360X.2021.00344

  • Tita I, Mogosanu GD, Tita MG. Ethnobotanical inventory of medicinal plants from the South West of Romania. Farmacia 2009; 57(2): 141– 156.

  • Von Kruedener S, Schneider W, Elstner EF. “A combination of Populustremula, Solidgovirgaurea and Fraxinus excelsior as an antiinflammatory and antirheumatic drug. A short review”. ArzneimittelForschung 1995; 45(2): 169–171. https://pubmed.ncbi.nlm.nih.gov/7710441/

  • Middleton P, Stewart F, Al-Qahtani S, Egan P, Orourke C, Abdulrahman A, Byres M, Middleton M, Kumarasamy Y, Shoeb M, Nahar L, Delazar A, Deysarker S. Antioxidant, antibacterial activities and general toxicity of Alnusglutinosa, Fraxinus excelsior and Papaver rhoeas. Iran. J Pharm Res 2005; (2): 81–86.

  • https://dx.doi.org/10.22037/ijpr.2010.620

  • Flanagan J, Meyer M, Pasamar MA, Ibarra A, Roller M, Genoher NAi, Leiva S, Gomez-García F, Alcaraz M, Martínez-Carrasco A, Vicente V. Safety evaluation and nutritional composition of a Fraxinus excelsior seed extract, FraxiPure™. Food and Chemical Toxicology 2013; 53:10–17.https://doi.org/10.1016/j.fct.2012.11.030

  • Okpanyi SN, Schirpke-von PR, Dickson D. Anti-inflammatory, analgesic and antipyretic effect of various plant extracts and their combinations in an animal model. Arzneimittel-Forshung 1989; 39(6):698–703.https://pubmed.ncbi.nlm.nih.gov/2476136/

  • Eddouks M, Maghrani M. Phlorizin-like effect of Fraxinus excelsior in normal and diabetic rats. J Ethnopharmacol. 2004; 94(1):149– 154.https://doi.org/10.1016/j.jep.2004.05.005

  • Eddouks M, Maghrani M, Zeggwagh NA, Haloui M, Michel J-B.Fraxinus excelsior L. evokes a hypotensive action in normal and spontaneously hypertensive rats. J Ethnopharmacol. 2005; 99(1):49– 54.https://doi.org/10.1016/j.jep.2005.01.050

  • Salman A, Muhammad MH, Zafar AM. Antiurolithiatic plants: Multidimensional pharmacology. Journal of Pharmacognosy and Phytochemistry 2016; 5(2): 04–24.

  • R. Kalai, B. Sasirekha. Dietary Guidelines to Reduce the Risk of Renal Stones. Int. J. Nur. Edu. and Research. 2018; 6(1): 73-77. doi: 10.5958/2454-2660.2018.00018.2

  • Flannigan R, Choy WH, Chew B, Lange D. Renal struvite stonespathogenesis, microbiology, and management strategies. Nat. Rev. Urol. 2014; 11(6):333–341. https://doi.org/10.1038/nrurol.2014.99

  • Manzoor MAP, Duwal SR, Mujeeburahiman M, Rekha P-D. Vitamin C inhibits crystallization of struvite from artificial urine in the presence of Pseudomonas aeruginosa. International Braz. J. Urol. 2018; 44(6):1234-1242. https://doi.org/10.1590/S1677-5538.IBJU.2017.0656

  • Chauhan CK, Joshi MJ, Vaidya ADB. Growth inhibition of Struvite crystals in the presence of herbal extract Commiphora wightii. Journalof Materials Science: Materials in Medicine 2009; 20(1): 85.https://doi.org/10.1007/s10856-008-3489-z

  • Torzewska A, Rozalski A. Inhibition of crystallization caused by Proteus mirabilis during the development of infectious urolithiasis by various phenolic substances. Microbiological research 2014; 169(7-8): 579-584.https://doi.org/10.1016/j.micres.2013.09.020

  • Vrunda Zalavadiya, Vipulshah, D.D. Santani. A Review on Urolithiasis and its Treatment using Plants. Research J. Pharmacognosy and Phytochemistry 2013; 5(1): 1-8.

  • Sujoy B, Aparna A. Potential clinical significance of urease enzyme.European Scientific Journal 2013; 9(21).

  • Kappaun K, Piovesan AR, Carlini CR, Ligabue-Braun R. Ureases: Historical aspects, catalytic, and non-catalytic properties. Journal of Advanced Research 2018; 13:3–17. https://doi.org/10.1016/j.jare.2018.05.010

  • Rieu P. Lithiases d’infection. Annales d’urologie 2005; 39:16–29. https://doi.org/10.1016/j.anuro.2005.01.001

  • Ramadevi S, Kaleeswaran B, Ilavenil S. Effect of traditionally used herb Pedalium murex L. and its active compound pedalitin on urease expression – For the management of kidney stone. Saudi Journal of Biological Sciences 2020; 27(3):833–839. https://doi.org/10.1016/j.sjbs.2020.01.014

  • K Geetha, R Manavalan, D Venkappaya. Effects and Causes of Lithiasis. Research J. Pharmacology and Pharmacodynamics. 2010; 2(4): 261-267

  • Daudon M, Jungers, P, Traxer O. Lithiase urinaire. Lavoisier, Médécine Sciences, Paris, 2012: 672p.

  • Falleh H, Ksoui R, Chaieb K, Karray-Bouraoui N, Trabelsi N, Boulaaba M, Abdelly C. Phenolic composition of Cynaracardunculus L. organs, and their biological activities. Compt Rend Biol2008; 331(5): 372-379.https://doi.org/10.1016/j.crvi.2008.02.008

  • Spandana. K, Shivani. M, Himabindhu. J, Ramanjaneyulu. K. Evaluation of In Vitro Antiurolithiatic Activity of Vignaradiata. Research J. Pharm. and Tech 2018; 11(12): 5455-5457. doi: 10.5958/0974-360X.2018.00994.0

  • Slinkard K, Singleton VL. Total phenol analyses: automation and comparison with manual methods. American Journal of Enology and Viticulture 1977; 28(1): 49-55.

  • Türkğluet A, Duru ME, Mercan N, Kivrak I, Gezer K. Antioxidant and antimicrobial activities of Laetiporussulphureus (Bull.) Murrill. a Food Chemistry 2007; 101(1):267–273.

  • https://doi.org/10.1016/j.foodchem.2006.01.025

  • Torzewska A, Stączek P, Różalski A. Crystallization of urine mineral components may depend on the chemical nature of Proteus endotoxin polysaccharides. Journal of Medical Microbiology 2003; 52(6):471– 477.https://doi.org/10.1099/jmm.0.05161-0

  • Prywer J, Ewa MB, Marcin O. Struvite crystal growth inhibition by trisodium citrate and the formation of chemical complexes in growth solution. Journal of Crystal Growth 2015; 418 :92– 101.https://doi.org/10.1016/j.jcrysgro.2015.02.027

  • Sadki C, Atmani F. Évaluation de l’effet antilithiasique, oxalo-calcique et phospho ammoniaco-magnésien d’extrait aqueux d’Erica multifloraL. Progrès en urologie 2017; 27(16) :1058– 1067.https://doi.org/10.1016/j.purol.2017.09.011

  • Daudon M, Protat MF, Réveillaud RJ. Analyse des calculs par spectrophotométrie infrarouge Avantages et limites de la méthode. Ann BiolClin1978; 36:475–489.

  • Swapnil R. Lahamage, Avinash B. Darekar, Ravindra B. Saudagar. Pharmaceutical Co-Crystallization. Asian J. Res. Pharm. Sci. 6(1): Jan.-Mar., 2016; Page 51-58. doi: 10.5958/2231-5659.2016.00008.4

  • Li H, Yao Q Z, Wang YY Li Y-L, Zhou G-T. Biomimetic synthesis of struvite with biogenic morphology and implication for pathological biomineralization. Scientific reports 2015; 5(1): 18.https://doi.org/10.1038/srep07718

  • El Habbani R, Lahrichi A, Sqalli Houssaini T, Kachkoul R, Mohim M, Chouhani BA and Chaqroune A. In vitro mass reduction of calcium oxalate urinary calculi by some medicinal plants. African Journal of Urology 2021; 27:28. https://doi.org/10.1186/s12301-021-00132-2

  • ChandakaMadhu, L. Harish, K. Narsimha Reddy, S. Sagar, D. Soumya, G. Pavan Kumar. Antiurolithic Activity of Aqueous Extract on Roots and Seeds of Plectranthustomentora on Ethylene Glycol Induced kidney Stones in Male Albino Rats. Asian J. Res. Pharm. Sci. 2(4): Oct.-Dec. 2012; Page 129-133.

  • N. V. Khokhlenkova, M. V. Buryak, O. V. Povrozina, T. V. Kamina. Principles of the Urolithiasis Phytotherapy. Research J. Pharm. and Tech 2019; 12(9):4559-4564. doi: 10.5958/0974-360X.2019.00784.4

  • Zhu H, Sun X, Lu J, Wang M, Fang Y and Ge W. The effect of plum juice on the prevention of struvite calculus formation in vitro BJU Intrnationa 2012; 110: E362-E367. https://doi.org/10.1111/j.1464410X.2012.11090.x

  • Chauhan CK, Joshi MJ. In vitro crystallization, characterization and growth inhibition study of urinary type struvite crystals. Journal of Crystal Growth 2013; 362: 330–337. https://doi.org/10.1016/j.jcrysgro.2011.11.008

  • Beghalia M, Belouatek A, Ghalem S, and Allali H. Inhibition and Dissolution Crystals of Magnesium Ammonium Phosphate by Acacia Radiana (Bark) and (Sheet) in Vitro Study Int'l Journal of Advances in

  • Agricultural & Environmental Engg 2014; 1: 1523-1531.http://dx.doi.org/10.15242/IJAAEE.C514072

  • Prywer J, Torzewska A. “Bacterially induced struvite growth from synthetic urine: experimental and theoretical characterization of crystal morphology”. Crystal Growth and Design 2009; 9(8): 3538–3543.

  • https://doi.org/10.1021/cg900281g

  • Jungers P, Daudon M, Le Duc A. Lithiase urinaire. Flammarion Médecine-Sciences 1989; 590 p.

  • Muryanto S, Sutanti S, Kasmiyatun M Inhibition of struvite crystal growth in the presence of herbal extract orthosiphonaristatus BL. MIQ. In MATEC Web of Conferences 2015; Vol. 58, p. 01013. EDP

  • Sciences.https://doi.org/10.1051/matecconf/20165801013

  • Chauhan CK, Joshi MJ, Vaidya ADB. Growth Inhibition of Struvite Crystals in the Presence of Herbal Extract Boerhaavia diffusa Linn. American Journal of Infectious Diseases 2009; 5 (3): 170– 179.https://doi.org/10.3844/ajidsp.2009.170.179

  • Scott MD, Andrew JR, Damien MB. Gastric patch pyeloplasty: development of an animal model to produce upper tract urinary acidification for treating struvite urinary calculi, J. Urol 2001; 166 (2):

  • –687. https://doi.org/10.1016/S0022-5347(05)66043-0

  • Prywer J, Torzewska A. Effect of Curcumin Against Proteus mirabilis During Crystallization of Struvite from Artificial Urine. EvidenceBased Complementary and Alternative Medicine 2012; Article ID

  • , 7 pages.https://doi.org/10.1155/2012/862794

  • In vitro Antiurolithiatic activity of the Leaves and Flowers extracts of Paronychia argentea, a plant used in Traditional medicine in Algeria. Omar Mechraoui, Ali Imessaoudene, Mohamed Y. Maiz, Hicham

  • Banouh, Lotfi Mouni, Abdelkrim Rebiai, Mohamed L. Belfar4, Noureddine Elboughdiri, Djamel Ghernaout, Bachir Ben Seghir. Asian J. Research Chem. 14(6): November – December 2021 DOI:10.52711/0974-4150.2021.00069

  • Kaleeswaran B, Ramadevi S, Murugesan R, Srigopalram S, Suman T, and Balasubramanian T. Evaluation of anti-urolithiatic potential of ethyl acetate extract of Pedalium murex L. on struvite crystal (kidney stone). Journal of Traditional and Complementary Medicine 2019; 9(1):24–37.https://doi.org/10.1016/j.jtcme.2017.08.003

  • Reshma R, Mahima V, Badrinathan S, Himaja M, Sabina EP, ArunaiNambi Raj N. In vitro and In vivo stady on the effect of ScopariaDulcis in inhibiting the growth of urinary crystals. International Journal of Phytomedcinie 2014; 6: 617–624.

  • Prywer J, Torzewska A Effect of curcumin against proteus mirabilis during crystallization struvite from artificial urine. J. Evid. Based Complementary Altern. Med 2012; 1–7.https://doi.org/10.1155/2012/862794

  • Dayana Jeyaleela G, Rosaline VimalaJ, Senthil R, Anandagopu P and ManjulaK. Isolation, Characterization, Molecular Docking and in vitro Studies of Inhibitory Effect on the Growth of Struvite Crystal Derived from Meliadubia Leaf Extract. Asian Journal of Chemistry 2019; 31(11): 2628-2634.https://doi.org/10.14233/ajchem.2019.22217


Abstract Views: 268

PDF Views: 0




  • Evaluation of the Antilithiasic activity, Phospho-ammoniaco-magnésien of the aqueous extract of Fraxinus excelsior (L.)

Abstract Views: 268  |  PDF Views: 0

Authors

Khaoula Ghenaiet
Laboratory of Biochemistry and Applied Microbiology, Badji Mokhtar Annaba University, 23000, Algeria., India
Abdelatif Boutefnouchet
Laboratory of Biophysics, Faculty of Medicine, Annaba, 23000, Algeria., India
Abdelatif Boutefnouchet
Research Unit Materials Science and Applications, Constantine University, 25000, Algeria., India
Asma Lekouaghet
Institute of Agronomic and Veterinary Sciences, Departement of Veterinary Medicine, University of Souk Ahras, 41000, Algeria., India
Nafissa Boutefnouchet Abassi
Institute of Agronomic and Veterinary Sciences, Departement of Veterinary Medicine, University of Souk Ahras, 41000, Algeria ., India
Lazhari Tichati
Environmental Research Center (ERC), Annaba, 23000, Algeria ., India

Abstract


The formation of urinary stones is a serious clinical problem in the world, the struvite of which is one of the characteristic components of urolithiasis of infectious origin. The objective of this study is to evaluate the in vitro litholytic and inhibitory effects of the leaf aqueous extract of Fraxinus excelsior L plant on the crystallization of struvite. The plant is commonly used in traditional Algerian medicine.A characterization method of Fraxinus excelsior L plant was first performed to determine the total phenolic and flavonoid compounds of Fraxinus excelsior L leaf aqueous extract. Struvite crystals prepared with two different processes had subsequently undergone an inhibition assay by supplementing Aqueous Extract with varying concentrations. In the first process, struvite was biologically crystallized in a synthetic urine in the presence of Proteus mirabilis during 24 hours of incubation period. This was followed by the addition of Aqueous Extract, the effect of which was evaluated after 1-hour. In the second process, struvite crystals were chemically synthesized for 30 minutes in the presence of aqueous extract. These two processes allow to assess, respectively, the litholytic effect of aqueous extract on the previously formed struvite crystals and the inhibitory effect of the crystallization of struvite. These various tests of crystallization and inhibition of struvite crystals were conducted by Polarized Light Microscopy (PLM) and Fourier Transform Infrared (FTIR) Spectroscopy. The results reveal that this Aqueous Extract contains an estimated total phenolic content of 60.92 ± 0.94 mg gallic acid equivalent (GAE)/g of extract and a total flavonoid content of 19.32 ± 0.75 mg quercetin equivalent (QE)/g of extract. The in vitro struvite crystals dissolution testing revealed a significant litholytic effect. Also, the struvite crystallization inhibition testing showed that it exerted a significant inhibitory effect observed with polarized light.The effect of this extract is evident against struvite crystals with a conclusive litholytic and inhibitory potential. The present results open up significant preventive management prospects for this type of lithiasis.

Keywords


Fraxinus excelsior, Urinary Lithiasis, Struvite; Litholytic Effect, Crystal Inhibition

References