Asian Journal of Research in Chemistry

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

RP-HPLC Method Development and Validation for Simultaneous Estimation of Levocetirizne Dihydrochloride and Phenylephrine hydrochloride in their tablet dosage form.


Affiliations
1 Sharad Pawar College of Pharmacy, Wanadongari, Nagpur(M.S.)
     

   Subscribe/Renew Journal


In this study, reverse phase high performance liquid chromatographic method has been developed and validated for the simultaneous determination of Levocetirizine and Phenylephrine in tablet dosage formulation. HPLC separation was achieved with Serveyor PDA/RI/FL model having Chromoquest 5 Software with 20.0 μl fixed loop injector, plus C18 (250mm×4.6mm, 5μm) column and UV detector as stationary phase and 10mM phosphate buffer (pH adjustedto 5.5) (80:20, v/v) as eluent, at a flow rate of 1.0ml/min. UV detection was performed at 278 nm. The retention time of Levocetirizine and Phenylephrine were found to 4.921 and 2.721min respectively. Results of the analysis were validated statistically and by recovery studies. The stability of Levocetirizine was maintained by carrying out all the operations in amber coloured glass wares with minimal exposure to light. The result of the studies showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which can be used for the routine determination of Levocetirizine and Phenylephrine in bulk and in its pharmaceutical dosage forms.

Keywords

Phenylephrine, Levocetirizine, HPLC, Validation
Subscription Login to verify subscription
User
Notifications
Font Size


  • Budhavari S. The Merk Index. 14th ed., Merk Research Lab. Division of Merck and Co. Inc. Whitehouse station NJ; 2001. p. 1011.

  • Budhavari S. The Merk Index. 14th ed. Merk Research Lab. Division of Merck and Co. Inc. Whitehouse station NJ; 2001. p. 1307.

  • Sweetman SC, Martin D. The Complete Drug Reference. 35th ed. Pharmaceutical Press, London; 2007. p. 526.

  • Indian Pharmacopoiea. Government of India Ministry of Health and Family Welfare;2007. Vol.2. p. 893.

  • British Pharmacopoeia. Int Ed.Published on the recommendation of the medicine commission pursuant to medicine act 1968; 1993.vol.1. p. 509.

  • Shende P, Shah V, Ghodke D. Validation of UV Spectrophotometric method for estimatiion of Levocetrizine dihydrochloride in bulk and pharmaceutical formulation. Journal of Pharmacy 2010; 3(10):2386-2387.

  • Merukar SS, Mhaskar PS, Bavaskar SR, Burade KB, Dhabale PN. Simultaneous spectrophotometric methods for estimation of Levocetrizine and Pseudoephidrine in Pharmaceutical Tablet Dosage Form. Journal of Pharmaceutical Sciences and Research 2009;1(2):3842.

  • Choudhari V, Kale A, Kuchekar B, Gawli V, Patil N. Simultaneous determination of Montelukast sodium and Levocveterizine dihydrochloride in pharmaceutical preparations by Ratio Derivative Spectroscopy. International Journal of Pharm Tech Research. March 2010;2(4):985-987.

  • P.I.Hair, L.J. Scott, Drugs 66(2006) 973.

  • G. Passalacqua, G.W. Canonica, Clin.Ther. 27 (2005) 979.

  • Swarbrick J, Boylan J. Encyclopedia of Pharmaceutical Technology, Vol. I, Marcel Dekker Inc. New York; 1998. p. 217 - 224.

  • Ebru ÇD, Mehmet G,Hale C.Validation of method for simultaneous determination of Paracetamol and Phenylephrine in pharmaceutical formulation by RP-LC method. International Journal of Comprehensive pharmacy 2011;6(09):231-232.

  • Hajare RA, Tated AG, Gadbail PA, Khan FA, Kayal SD.Method development and validation for simultaneous determination of Ebastine and Phenylephrine HCl in tablet formulation by RPHPLC. International Journal of Pharmaceutical Research and development 2011;3(7):214-220.

  • Ashokkumar S, Senthil Raja M, Perumal P. RP-HPLC Method Development and Validation for Simultaneous Estimation of Montelukast Sodium and Levocetrizine. Journal of Pharmaceutical Research 2009; 1(4): 8-12.


Abstract Views: 414

PDF Views: 2




  • RP-HPLC Method Development and Validation for Simultaneous Estimation of Levocetirizne Dihydrochloride and Phenylephrine hydrochloride in their tablet dosage form.

Abstract Views: 414  |  PDF Views: 2

Authors

Urwashi D. Ramteke
Sharad Pawar College of Pharmacy, Wanadongari, Nagpur(M.S.)
S. P. Wate
Sharad Pawar College of Pharmacy, Wanadongari, Nagpur(M.S.)

Abstract


In this study, reverse phase high performance liquid chromatographic method has been developed and validated for the simultaneous determination of Levocetirizine and Phenylephrine in tablet dosage formulation. HPLC separation was achieved with Serveyor PDA/RI/FL model having Chromoquest 5 Software with 20.0 μl fixed loop injector, plus C18 (250mm×4.6mm, 5μm) column and UV detector as stationary phase and 10mM phosphate buffer (pH adjustedto 5.5) (80:20, v/v) as eluent, at a flow rate of 1.0ml/min. UV detection was performed at 278 nm. The retention time of Levocetirizine and Phenylephrine were found to 4.921 and 2.721min respectively. Results of the analysis were validated statistically and by recovery studies. The stability of Levocetirizine was maintained by carrying out all the operations in amber coloured glass wares with minimal exposure to light. The result of the studies showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which can be used for the routine determination of Levocetirizine and Phenylephrine in bulk and in its pharmaceutical dosage forms.

Keywords


Phenylephrine, Levocetirizine, HPLC, Validation

References