Asian Journal of Research in Chemistry

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Equilibrium, Kinetics, DNA Binding and Antimicrobial studies of Trans Amine Cobalt (III) Ethylene Diamine Complex


Affiliations
1 Department of Chemistry, Osmania University, Hyderabad., Andhra Pradesh, PIN-500 007, India
     

   Subscribe/Renew Journal


Trans- [ICH2Co(en)2NH3] Complex was synthesized and characterized by chemical analysis, 1H, 13C NMR and Mass spectra. Pseudo first order reaction kinetics and binding studies of trans- [ICH2Co(en)2NH3] complex with imidazole, substituted imidazoles, histidine, histamine, glycine and ethyl glycine ester has been investigated using spectrophotometric technique. Equilibrium constants were determined as a function of pH at 25°c. Binding and kinetic studies were correlated based on basicity. steric hindrance. From the equilibrium data, it was found that the entering nucleophile participates in the transition state, thereby SN1 mechanism was proposed. The effect of the incoming ligands on the complex was studied by molecular mechanics. The binding property of this complex with DNA was observed using UV/Visible, Fluorescence studies. Antimicrobial property of this complex was also studied using E. coli bacteria.

Keywords

Bioactive Compounds, DNA-binding, VitaminB12 Models
Subscription Login to verify subscription
User
Notifications
Font Size


  • Brown K.L. Dalton Trans, 2006, 1123

  • Randiccio L. Comments Inorg. Chem, 1999, 21, 327.

  • P. Kofod, Inorg.Chem. 1995, 34, 2768.

  • ‘Vitamin B12 and B12-Proteins’, Eds: B. Kräutler, D. Arigoni, B. T Golding, Wiley-VCH, Weinheim, Germany, 1998.

  • D. Dolphin, A. W. Johnson, Chem. Commun. (London) 1965, 494.

  • K. Farmery, D. H. Busch, Inorg Chem, 1972, 11, 2901.

  • G. N. Schrauzer, Angew. Chem., Int. Ed. 1976, 15, 417; B. D. Gupta, V. Vijaikanth, V. Singh, Organometallics, 2004, 23, 2069; V. Vijaikanth, B. D. Gupta, D. Mandal, S. Shekhar, Organometallics 2005, 24, 4454.

  • G. Costa, G. Mestroni, E. de Savorgnami, Inorg. Chim. Acta 1969, 3, 323.

  • a) T. S. Roche, J. F. Endicott, J. Am. Chem. Soc. 1972, 94, 8622.; b) P. Kofod, P. Harris, S. Larsen, Inorg.Chem. 1997, 36, 2258.

  • F. Asaro, L. Liguori, G. Pellizer, Phys. Chem. Chem. Phys. 1999, 1, 4981.

  • L. Randaccio, N. Bresciani-Pahor, E. Zangrando, L. G. Marzilli, Chem. Soc. Rev. 1989, 18, 225.

  • D. R. Sudharshan Reddy, B.. Krishna, P. Pallavi, N. Navaneetha, S. Satyanarayana, Indian. J. of Chem. 2005, 44A, 678.

  • N. Navaneetha, S. Satyanarayana, Indian J. of Chem. 2005, 44A, 1191.

  • J. V. Madhuri, V. Malathi, S. Satyanarayana, J. of Chem. Sci. 2004, 116, 143.

  • J.V. Madhuri, V. Malathi, S. Satyanarayana, J. of Chem. Sci. 2005, 117, 305.

  • K. G. Meyer, Synlett 2004, 2355.

  • C. K. Poon, Coord. Chem. Rev. 1973, 10, 1.

  • A. W. Herlinger, T. L. Brown, J. Am. Chem. Soc. 1972, 94, 388.

  • P. Daublain, J. H. Horner, A. Kuznetsov, M. Newcomb, J. 20. J. Zsako, Z. Finta, C. S. Varhelyl, J. Inorg. Nucl. Chem. 1972, 34, 2887.

  • N. Fey, J. Chem. Technol. Biotechnol. 1999, 74, 852.

  • I. B. Bersukker, M. K. Leong, J. E. Boggs, R. S. Pearl Man, Bull. Soc. Chil. Quim 1997, 42, 405. R. Cini, G. Giorgi, F. Laschi, C. Rossi, L. G. Marzilli, J. Biomol. Struct. Dyn. 1990, 7, 859.

  • N. L. Allinger, J. Am. Chem. Soc. 1977, 99, 8127.

  • J. M. Kelly, A. B. Tossi, D. J. McConnell, C. OhUigin, Nucleic Acids Res. 1985, 13, 6017.

  • S. A. Tysoe, R. J.Morgan, A. D. Baker, T. C. Strekas, J.Phys.Chem. 1993, 97, 1707.

  • A. Wolfe, G. H. Shimer , T. Meehan, Biochemistry, 1987, 26, 6392.

  • Ponuganti Pallavi, Penumaka Nagababu, and Sirasani Satyanarayana, Helvicta Chimica Acta, 2007, 90, 627-639.

  • J.R. lakowicz, priciples of Fluorescence Spectroscopy, Plenum Press, 5523.

  • D.S. Sigman, 1986, Acc.Chem. Res, 19, 180- 186.

  • S.Sitlani, E. C.Long, A.M.Pyle, J.K. Barton, 1992, J.Am.Chem.Soc. 114, 2303- 2312.


Abstract Views: 357

PDF Views: 2




  • Equilibrium, Kinetics, DNA Binding and Antimicrobial studies of Trans Amine Cobalt (III) Ethylene Diamine Complex

Abstract Views: 357  |  PDF Views: 2

Authors

Padmaja Naishadham
Department of Chemistry, Osmania University, Hyderabad., Andhra Pradesh, PIN-500 007, India
S Satyanarayana
Department of Chemistry, Osmania University, Hyderabad., Andhra Pradesh, PIN-500 007, India

Abstract


Trans- [ICH2Co(en)2NH3] Complex was synthesized and characterized by chemical analysis, 1H, 13C NMR and Mass spectra. Pseudo first order reaction kinetics and binding studies of trans- [ICH2Co(en)2NH3] complex with imidazole, substituted imidazoles, histidine, histamine, glycine and ethyl glycine ester has been investigated using spectrophotometric technique. Equilibrium constants were determined as a function of pH at 25°c. Binding and kinetic studies were correlated based on basicity. steric hindrance. From the equilibrium data, it was found that the entering nucleophile participates in the transition state, thereby SN1 mechanism was proposed. The effect of the incoming ligands on the complex was studied by molecular mechanics. The binding property of this complex with DNA was observed using UV/Visible, Fluorescence studies. Antimicrobial property of this complex was also studied using E. coli bacteria.

Keywords


Bioactive Compounds, DNA-binding, VitaminB12 Models

References