Asian Journal of Research in Chemistry

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Development and Validation of HPTLC Method for Quantification of Gallic acid and Catechin from Draksharishta


Affiliations
1 Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, India
2 Department of Pharmaceutical Chemistry, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, India
3 Head of Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, India
     

   Subscribe/Renew Journal


A simple, precise and accurate HPTLC method has been established for the determination of gallic acid and catechin in test formulations of Draksharishta as Draksharishta-T and Draksharishta-M prepared by traditional and modern method respectively and also in its marketed formulation. Draksharishta is a polyherbal hydro alcoholic preparation and is used to improve digestion, as blood purifier, in the treatment of anaemia and advised as a choice of remedy in respiratory problems. The developed HPTLC method was validated in terms of precision, accuracy, LOD, LOQ and specificity. The amount of gallic acid in Draksharishta-T, M and in its marketed formulation was found to be 0.0227, 0.0225 and 0.0226 % w/w respectively while catechin was found to be 0.0176, 0.0175 and 0.0175 %w/w respectively. Quantification of gallic acid and catechin in Draksharishta has been reported first time by validated HPTLC. Furthermore, no TLC densitometric methods have been reported for the quantification of gallic acid and catechin from Draksharishta.

Keywords

Draksharishta, HPTLC, Validation, Gallic Acid, Catechin
Subscription Login to verify subscription
User
Notifications
Font Size


  • The Ayurvedic Formulary of India Part-I, Controller of Publications, Delhi; 2000:15-16.

  • Baydar NG, Ozkan G, Sagdic O. Total phenolic contents and antibacterial activities of grape (Vitis vinifera L.) extracts. Food Control 2004; 15: 335-339.

  • Frankel EN, Kanner J, German JB, parks E, Kinsella JE. Inhibition of oxidation of human low density lipoprotein by phenolic substances in red wine. Lancet 1993; 341 (20): 454-457.

  • Mayer AS, Yi OS, Person DA, waterhouse DL, Frankel EN. Inhibition of human low density lipoprotein oxidation in relation to composition of phenolic antioxidants in grapes (Vitis vonofera). Journal of Agriculture and Food Chemistry 1997; 45: 1638-1643.

  • Teissure PL, Frankel EN, Waterhouse AL, Peleg H, German GB. Inhibition of in vitro human LDL oxidation by phenolic antioxidants from grapes and wines. Journal of Science Food and Agriculture 1996; 70:55-61.

  • Waterhouse AL. Wine antioxidants may reduce heart disease and cancer. Presentation of American Chemical Society, Washington; 1994.

  • Mishra S, Bhaisazya Kalpana Vigyan. Varanasi (India): Chaukambha Surbharati Prakashan; 2005:253-254.

  • Alam M, Radhamani S, Ali U, Puroshottam KK. Microbiological screening of dhataki flowers. Journal of research in Ayurveda and Siddha 1984; 2(4): 371-375.

  • ICH guidelines Q2 (R1). Validation of analytical procedures. Methodology, Geneva, 2006.

  • Singh B, Mungara P, Nivsarkar M, Anandjiwala S. HPTLC densitometry quantification of glycyrrhizin, glycyrrhizinic acid, apigenin, kaempferol and quercetin from Glycyrrhiza glabra. Chromatographia 2009; 70: 1665-1672.

  • R.P.W. Scott, Encyclopedia of Chromatography, 10th edn., Marcel Dekker, USA, 2001, p.p. 252-254.


Abstract Views: 371

PDF Views: 1




  • Development and Validation of HPTLC Method for Quantification of Gallic acid and Catechin from Draksharishta

Abstract Views: 371  |  PDF Views: 1

Authors

Preeti Tiwari
Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, India
D.J. Sen
Department of Pharmaceutical Chemistry, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, India
Rakesh K. Patel
Head of Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, India

Abstract


A simple, precise and accurate HPTLC method has been established for the determination of gallic acid and catechin in test formulations of Draksharishta as Draksharishta-T and Draksharishta-M prepared by traditional and modern method respectively and also in its marketed formulation. Draksharishta is a polyherbal hydro alcoholic preparation and is used to improve digestion, as blood purifier, in the treatment of anaemia and advised as a choice of remedy in respiratory problems. The developed HPTLC method was validated in terms of precision, accuracy, LOD, LOQ and specificity. The amount of gallic acid in Draksharishta-T, M and in its marketed formulation was found to be 0.0227, 0.0225 and 0.0226 % w/w respectively while catechin was found to be 0.0176, 0.0175 and 0.0175 %w/w respectively. Quantification of gallic acid and catechin in Draksharishta has been reported first time by validated HPTLC. Furthermore, no TLC densitometric methods have been reported for the quantification of gallic acid and catechin from Draksharishta.

Keywords


Draksharishta, HPTLC, Validation, Gallic Acid, Catechin

References