Asian Journal of Research in Chemistry

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Spectrophotometric method for the determination of Vitamin A and E using Ferrozine-Fe(II) complex


Affiliations
1 Department of Natural Resources Engineering and Management, University of Kurdistan, Hewler, Iraq
2 Department of IMBB, University of Lahore, Pakistan
3 Department of Chemistry, University of Balochistan, Quetta, Pakistan
     

   Subscribe/Renew Journal


A simple and sensitive flow injection Spectrophotometric method is reported for the determination of vitamin A and E using Ferrozine-Fe(II) detection system. In the presence of vitamin A/E, Fe(III) reduces. The in situ reduced ions are then reacted with Ferrozine to make a magenta colored complex, which is monitored at absorption wavelength of 562 nm. Vitamin A shows a linear calibration graph in the concentration range of 0.1-10 μg/mL with a limit of detection (3s) of 0.06 μg/mL. The coefficient of determination was r2=0.9974 with relative standard deviations (n = 4) in the range of 0.8-2.8%. The proposed method allowed 10 injections per hour. Vitamin E gives a calibration graph linear in the concentration range of 0.1-20 μg/mL with a limit of detection (3s) of 0.03 μg/mL. The coefficient of determination was r2=0.9993 with relative standard deviations (n = 4) in the range of 1.1- 2.6% with a sample throughput of 30 injections per hour. The method was applied to determine both vitamins in pharmaceuticals, infant milk and blood serum samples using hexane extraction. The method is validated by comparing with HPLC reference method. The calculated results showed that there is no significant difference between the two methods at 95% confidence level.

Keywords

Spectrophotometery, Vitamin A And E, Fe (ll), Ferrozine, Pharmaceutical, Blood Serum
Subscription Login to verify subscription
User
Notifications
Font Size


  • Amin, A. S. 2001. Colorimetric determination of tocopheryl acetate (vitamin E) in pure form and in multivitamin capsules. Eur. J. Pharm. Biopharm. 51(3): 267–272.

  • Asgher M, Yaqoob M, Waseem A, Nabi A. Flow injection methods for the determination of Retinol and ?-Tocopherol using lucigenin enhanced chemiluminescence. Luminescence, 26(6) (2011) 416–423.

  • Combs G.F. Jr., The Vitamins: Fundamental Aspects in Nutrition and Health, Elsevier Academic Press, USA, (2008).

  • Devi I, Memons. A., Khuhawar M.Y. Spectrophotometric analysis of Vitamin E using Cu (l)-Bathocuproine,and Fe(ll)- 2,4,6 tris-(2-pyridyl)tarizine complexes. Journal of the Chemical Society of Pakistan, 2004, 26(3), 239-243.

  • Driskell, W. J., W. J. Neese, C. C. Bryant, and M. M. Bashor. 1982. Measurement of vitamin A and vitamin E in human serum by high-performance liquid chromatography. J. Chromatogram. B 231(2): 439–444.

  • Fu P, Xia Q, Boudreau MD, Howard PC, Tolleson W, Wamer W. Physiological role of retinyl palmitate in the skin. Vitamins and Hormones – Vitamin A, 75:223–56.

  • Helrich K, Official Methods of Analysis of the Association of Official Analytical Chemists, Washington, DC, 15th ed., p. 1070.

  • Hoppe PP, Krennrich G. Bioavailability and potency of naturalsource and all-recemic -tocopherol in human: a dispute. Euro J. Nutr. 2000, 39, 183-93.

  • Jadoon S, Waseem A, Yaqoob M and Nabi A. Flow injection Spectrophotometric determination of vitamin E in pharmaceuticals, milk powder and blood serum using potassium ferricyanide-Fe(III) detection system. Chinese Chemical Letters, 21(6) (2010) 712–715.

  • Khalid A, Waseem A, Afzal I, Yaqoob M, Nabi A, Yasinzai MM. Flow Injection Spectrophotometric and Spectrofluorimetric methods for the Determination of Candesartan Cilexetil in Pharmaceuticals. Scientific Research and Essays. 6(29) (2011) 6203–6208.

  • Kienen, V., W. F. Costa, J. V. Visentainer, N. E. Souza, and C. C. Oliveira. 2008. Development of a green chromatographic method for determination of fat-soluble vitamins in food and pharmaceutical supplement. Talanta 75(1): 141–146.

  • Mikheeva E.V., Anisimova L.S. Voltammetric determination of vitamin E (-Tocopherol acetate) in multicomponent vitaminized mixtures. J. Anal. Chem. 62 (2007) 373.

  • Prieto P., Pineda M., Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal. Biochem. 269 (1999) 337.

  • Razagui, I. B., P. J. Barlow, and K. D. A. Taylor. 1992. A spectrofluorimetric procedure for the determination of atocopherol in nutritional supplement products. Food Chem. 44(3): 221–226.

  • Rishi L, Jadoon S, Waseem A., Yaqoob M., Nabi A. Flowinjection methods for the determination of -tocopherol with spectrophotometric detection. Journal of The Chemical Society of Pakistan, 33(4) (2011) 508–514.

  • Semeraro, A. I., M. Altieri, Patriarca, and A. Menditto. 2009. Evaluation of uncertainty of measurement from method validation data: An application to the simultaneous determination of retinol and a-tocopherol in human serum by HPLC. J. Chromatogr. B 877(11–12): 1209–1215.

  • Siluk D, Oliveira RV, Esther-Rodriguez-Rosas M, Ling S, Bos A, Ferrucci L, Irving W. Wainer. A validated liquid chromatography method for the simultaneous determination of vitamins A and E in human plasma. J Pharm Biomed Anal. 2007, 15; 44(4): 1001–1007.

  • Tutem E., Apak R., Gunaydi E., Sözgen K. Spectrophotometric determination of vitamin E (-tocopherol) using copper(II)- neocuproine reagent Talanta 44 (1997) 249.

  • Waseem A, Rishi L, Yaqoob M and Nabi A. A Flow-Injection determination of Cysteine, N-Acetyl cysteine and Glutathione in Pharmaceuticals using Potassium Ferricyanide-Fe (III) Spectrophotometric system. Chemical Research in Chinese Universities, 26(6) (2010) 893–898.

  • Waseem A, Rishi L, Yaqoob M and Nabi A. Flow Injection Determination of Retinol and Tocopherol in Pharmaceuticals with acidic Potassium permanganate Chemiluminescence. Analytical Sciences, 25(3) (2009) 407-412.

  • Waseem A, Yaqoob M and Nabi A, Hussain I. Flow-Injection Spectrophotometric procedure for the determination of Uric Acid in Urine using Prussian blue reaction. Chemical Research in Chinese Universities 27(6) (2011) 929–933.

  • Yaqoob M, Waseem A and Nabi A. Flow-Injection Determination of Vitamin A in Pharmaceuticals Formulations using Tris(2,2'- bipyridyl)Ru(II)-Ce(IV) Chemiluminescence Detection. Luminescence 24(5) (2009) 276-281.

  • Yinfa, M. 1996. Quantitative retinol assay for serum and dried blood spots. US Patent 5532166


Abstract Views: 825

PDF Views: 2




  • Spectrophotometric method for the determination of Vitamin A and E using Ferrozine-Fe(II) complex

Abstract Views: 825  |  PDF Views: 2

Authors

Saima Jadoon
Department of Natural Resources Engineering and Management, University of Kurdistan, Hewler, Iraq
Arif Malik
Department of IMBB, University of Lahore, Pakistan
M.H. Qazi
Department of IMBB, University of Lahore, Pakistan
Muhammad Aziz
Department of Chemistry, University of Balochistan, Quetta, Pakistan

Abstract


A simple and sensitive flow injection Spectrophotometric method is reported for the determination of vitamin A and E using Ferrozine-Fe(II) detection system. In the presence of vitamin A/E, Fe(III) reduces. The in situ reduced ions are then reacted with Ferrozine to make a magenta colored complex, which is monitored at absorption wavelength of 562 nm. Vitamin A shows a linear calibration graph in the concentration range of 0.1-10 μg/mL with a limit of detection (3s) of 0.06 μg/mL. The coefficient of determination was r2=0.9974 with relative standard deviations (n = 4) in the range of 0.8-2.8%. The proposed method allowed 10 injections per hour. Vitamin E gives a calibration graph linear in the concentration range of 0.1-20 μg/mL with a limit of detection (3s) of 0.03 μg/mL. The coefficient of determination was r2=0.9993 with relative standard deviations (n = 4) in the range of 1.1- 2.6% with a sample throughput of 30 injections per hour. The method was applied to determine both vitamins in pharmaceuticals, infant milk and blood serum samples using hexane extraction. The method is validated by comparing with HPLC reference method. The calculated results showed that there is no significant difference between the two methods at 95% confidence level.

Keywords


Spectrophotometery, Vitamin A And E, Fe (ll), Ferrozine, Pharmaceutical, Blood Serum

References