Asian Journal of Research in Chemistry

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Development and Validation of RP-HPLC Method for Simultaneous Estimation of Eperisone Hydrochloride and Lornoxicam in Synthetic Mixture


Affiliations
1 Department of Quality Assurance, S.K. Patel College of Pharmaceutical Education & Research, Ganpat University, Ganpat Vidyanagar – 384012, Mehsana, Gujarat, India
     

   Subscribe/Renew Journal


A simple, accurate, and rapid reversed phase high performance liquid chromatographic method has been developed and validated for the simultaneous estimation of Eperisone Hydrochloride (EPE) and Lornoxicam (LOR) in synthetic mixture. The separation was carried out using mobile phase consisting of methanol: ACN: water (60: 30: 10, v/v/v) (pH-3, adjusted with OPA). The column used was Phenomenex C18, (250 mm x 4.6 mm i.d., 5 μm) with flow rate 1 ml/min using PDA detection at 255 nm. The method was linear over a concentration range 10 - 100 μg/ml for EPE and 2 - 20 μg/ml for LOR. The retention time of Eperisone hydrochloride and Lornoxicam were found to be 2.2 min and 3.15 min respectively. Results of analysis were validated statistically and by recovery studies. The mean recovery was 99.07 ± 0.42 and 100.79 ± 1.05 for EPE and LOR respectively. The limit of detection (LOD) and the limit of quantification (LOQ) for EPE and LOR were found to be 0.3504 and 1.0618 μg/ml and 0.1903 and 0.5769 μg/ml, respectively. The results of the study showed that the proposed RP-HPLC method was found to be simple, sensitive, precise and accurate and also useful for the routine determination of EPE and LOR in mixture.

Keywords

Eperisone Hydrochloride, Lornoxicam, RP-HPLC, Validation
Subscription Login to verify subscription
User
Notifications
Font Size


  • Maryadele J O Neil. The Merck Index: An Encyclopedia of chemicals, drugs and biologicals, 14th edition. New Jersey: Published by Merck Research Laboratories, Division of Merck and Co., Inc. Whitehouse station, 2006, p.610.

  • The Japanese Pharmacopoeia, society of Japanese Pharmacopoeia, 15th edition, Shibuya Tokyo Japan, 2006, p.618.

  • Jeoung M, Jeong E, Kim N, Kim C, Chung Y, Lee Y, Ahn S, Cho H, Lee Y, Hong J, Moon D. Determination of Eperisone in human plasma by Liquid chromatography-ESI-tandem mass spectrometry. Arch Pharm Research, 30(9), 2007, p.1174-1178

  • Ding L, Wei X, Zhang S, Sheng J, and Zhang Y. Rapid and Sensitive Liquid Chromatography–Electrospray Ionization- Mass Spectrometry Method for the Determination of Eperisone in Human Plasma. Journal of Chromatographic Science, 42(5). 2004, p.254-258.

  • Ding L, Wang X, Yang Z, and Chen Y. The use of HPLC/MS, GC/MS, NMR, UV and IR to Identify Degradation product of Eperisone hydrochloride in the tablets, Journal of pharm and Bio Anal, 46, 2008, p.282-287.

  • Maryadele J O Neil. The Merck Index: An Encyclopedia of chemicals, drugs and biologicals, 14th edition. New Jersey: Published by Merck Research Laboratories, Division of Merck and Co., Inc. Whitehouse station, 2006, p.5582

  • Devanaboyina N, Satyanarayana T, Rao B. A novel RP-HPLC method for the quantification of lornoxicam in formulation. Journal of Pharmacy Research, 4(12), 2011, p.4741-4743.

  • Shital B, Nikhil K. Extractionless High-Performance Liquid Chromatographic method for determination of lornoxicam in human plasma. Asian Journal of Pharmaceutical and Clinical Research, 5(1), 2012, p.122-124.

  • Sunit S, Ranjit G, Sachin P, Amulya B, Ranjit M. Development of Ultraviolet Spectrophotometric Method for Analysis of Lornoxicam in Solid Dosage Forms. Tropical Journal of Pharmaceutical Research, 11(2), 2012, p.269-273.

  • Shital B, Santosh G, Padmanabh D, Navjot G. High performance thin layer chromatographic determination of lornoxicam in human plasma. Journal of Chemical, Biological and Physical Sciences, 2(1), 2011-2012, p.279-283.

  • Zeng L, Chen Y, Zhang F, Zhong F. Determination of Lornoxicam in human plasma by LC/MS/MS. Pubmed,39(2), 2004, p.132-135.

  • Rele V, Warkar B. Estimation of Lornoxicam and Diacerin in dosage form by Simultaneous equation & Q-analysis method using UV Spectroscopic technique. International Journal of Pharma and Bio Sciences, 2(2), 2011, p.124-129.

  • Pankaj K, Shubhanjali S, B Subudhia, Ashok G. Bioanalytical Method development & Validation for the Simultaneous estimation of Thiocolchicoside &Lornoxicam in Human plasma and in Pharmaceutical dosage form by RP-HPLC. International Journal of Pharmacy and Pharmaceutical Sciences, 4(3), 2012, p.252-259.

  • The International Conference on Harmonization, Q2 (R1), Validation of Analytical Procedure, Text and Methodology, 2005.


Abstract Views: 453

PDF Views: 2




  • Development and Validation of RP-HPLC Method for Simultaneous Estimation of Eperisone Hydrochloride and Lornoxicam in Synthetic Mixture

Abstract Views: 453  |  PDF Views: 2

Authors

Sejal K. Patel
Department of Quality Assurance, S.K. Patel College of Pharmaceutical Education & Research, Ganpat University, Ganpat Vidyanagar – 384012, Mehsana, Gujarat, India
Harshil R. Patel
Department of Quality Assurance, S.K. Patel College of Pharmaceutical Education & Research, Ganpat University, Ganpat Vidyanagar – 384012, Mehsana, Gujarat, India

Abstract


A simple, accurate, and rapid reversed phase high performance liquid chromatographic method has been developed and validated for the simultaneous estimation of Eperisone Hydrochloride (EPE) and Lornoxicam (LOR) in synthetic mixture. The separation was carried out using mobile phase consisting of methanol: ACN: water (60: 30: 10, v/v/v) (pH-3, adjusted with OPA). The column used was Phenomenex C18, (250 mm x 4.6 mm i.d., 5 μm) with flow rate 1 ml/min using PDA detection at 255 nm. The method was linear over a concentration range 10 - 100 μg/ml for EPE and 2 - 20 μg/ml for LOR. The retention time of Eperisone hydrochloride and Lornoxicam were found to be 2.2 min and 3.15 min respectively. Results of analysis were validated statistically and by recovery studies. The mean recovery was 99.07 ± 0.42 and 100.79 ± 1.05 for EPE and LOR respectively. The limit of detection (LOD) and the limit of quantification (LOQ) for EPE and LOR were found to be 0.3504 and 1.0618 μg/ml and 0.1903 and 0.5769 μg/ml, respectively. The results of the study showed that the proposed RP-HPLC method was found to be simple, sensitive, precise and accurate and also useful for the routine determination of EPE and LOR in mixture.

Keywords


Eperisone Hydrochloride, Lornoxicam, RP-HPLC, Validation

References