Asian Journal of Research in Chemistry

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Development and Validation of UV-Spectrophotometric Method for Determination of Cephalexin


Affiliations
1 Department of Pharmaceutical Analysis, Annamacharya college of Pharmacy, Rajampet, Kadapa Dist, A.P, India
     

   Subscribe/Renew Journal


To develop simple, sensitive, selective and accurate UV Spectrophotometric methods for the determination of Cephalexin in bulk drug and pharmaceutical formulations (Capsules). The absorbances were measured at 261(Method A) and 257 nm (Method B). The methods was found to be linear from a quantitation ranges of 5μg/ml to 40μg/ml (Method A) in Phosphate Buffer pH 2.0 and 5μg/ml to 50μg/ml (Method B) in 0.1 N HCl. The regression of the curves was Y = 0.020x - 0.021 (Method A) and Y = 0.014x - 0.102 (Method B). The methods gave satisfactory results in terms of repeatability and intermediate precision (RSD<1.010%) (Method A) and (RSD<0.589%) (Method B) 0.855 μg/mL and 2.85 μg/mL (Method A) and 2.357 μg/mL and 7.857 μg/mL were the LOD and LOQ values, respectively. The methods was validated and proved to be robust and rugged. The results of analysis for these methods have been validated statistically and by recovery studies.

Keywords

Cephalexin, Spectroscopic Method, Phosphate Buffer (Ph 2.0), 0.1 N Hcl
Subscription Login to verify subscription
User
Notifications
Font Size


  • The United States Pharmacopoeia XXIII. 1993. United States Pharmacopoeia Convection. Rockville, Maryland, U. S. A.

  • Agbaba D. et al., HPTLC assay of cephalexin and cefaclor in pharmaceuticals. Biomedical Chromatography, 1998, 12, 133- 135.

  • Halkar U.P. and Bhandari N.P. Simultaneous determination of cephalexin and probenecid in pharmaceutical preparations by HPTLC. Indian Drugs, 1998, 35, 382–383.

  • Coran S.A. et. al., Development of a densitometry method for the determination of cephalexin as an alternative to the standard HPLC procedure. Journal of Pharmaceutical and Biomedical Analysis., 1998, 18, 271–274.

  • Bhooshan R. and Prashad V., Separation and Identification of Some Cephalosporin’s on Impregnated TLC Plates., Biomedical Chromatography, 1996, 10, 258–260.

  • Najib N. M. et. al., High performance liquid chromatographic analysis of cephalexin in serum and urine., Journal of Clinial pharmacy and therapeutics, 2008, 12, 419-426.

  • Zendelovska D. et. Al., Simultaneous quantification of cefaclor and cephalexine in bloodplasma using high-performance liquid chromatography with UV detection., Acta Pharm,2002, 52, 243– 250.

  • Lee Y.J. and Lee H.S., Simultaneous determination of cefoxitin, cefuroxime, cephalexin and cephaloridine in plasma using HPLC and a column-switching technique. Chromatographia, 1990, 30, 80–84.

  • Prayanka P. and Suresh P., Development of colorimetric method for cephalexin in dosage forms, Asian Journal of Pharmaceutics, 2008, 2, 120–122.

  • Patel S.A. et. al., Spectrophotometric methods for the estimation of Cephalexin in tablet dosage forms., Indian Journal of Pharmaceutical Sciences, 2006, 68, 278-280.

  • Validation of Analytical Procedures: Text And Methodology Q1A (R2), ICH Harmonized Tripartite Guideline, Feb 2003.

  • Green J M, A practical guide to analytical method validation, Analytical Chemistry: 1996, 305A.


Abstract Views: 644

PDF Views: 2




  • Development and Validation of UV-Spectrophotometric Method for Determination of Cephalexin

Abstract Views: 644  |  PDF Views: 2

Authors

G. Venkata Prasad
Department of Pharmaceutical Analysis, Annamacharya college of Pharmacy, Rajampet, Kadapa Dist, A.P, India
S. Sravani
Department of Pharmaceutical Analysis, Annamacharya college of Pharmacy, Rajampet, Kadapa Dist, A.P, India
B. Mohammed Ishaq
Department of Pharmaceutical Analysis, Annamacharya college of Pharmacy, Rajampet, Kadapa Dist, A.P, India
M. Madhu
Department of Pharmaceutical Analysis, Annamacharya college of Pharmacy, Rajampet, Kadapa Dist, A.P, India
Sreenivasulu Munna
Department of Pharmaceutical Analysis, Annamacharya college of Pharmacy, Rajampet, Kadapa Dist, A.P, India
C. Gopinath
Department of Pharmaceutical Analysis, Annamacharya college of Pharmacy, Rajampet, Kadapa Dist, A.P, India

Abstract


To develop simple, sensitive, selective and accurate UV Spectrophotometric methods for the determination of Cephalexin in bulk drug and pharmaceutical formulations (Capsules). The absorbances were measured at 261(Method A) and 257 nm (Method B). The methods was found to be linear from a quantitation ranges of 5μg/ml to 40μg/ml (Method A) in Phosphate Buffer pH 2.0 and 5μg/ml to 50μg/ml (Method B) in 0.1 N HCl. The regression of the curves was Y = 0.020x - 0.021 (Method A) and Y = 0.014x - 0.102 (Method B). The methods gave satisfactory results in terms of repeatability and intermediate precision (RSD<1.010%) (Method A) and (RSD<0.589%) (Method B) 0.855 μg/mL and 2.85 μg/mL (Method A) and 2.357 μg/mL and 7.857 μg/mL were the LOD and LOQ values, respectively. The methods was validated and proved to be robust and rugged. The results of analysis for these methods have been validated statistically and by recovery studies.

Keywords


Cephalexin, Spectroscopic Method, Phosphate Buffer (Ph 2.0), 0.1 N Hcl

References