Asian Journal of Research in Chemistry

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Dipeptidylpeptidase-4 Inhibitory Activity of Pergularia Daemia (forsk) - An In-vitro Estimation


Affiliations
1 Department of Pharmacology, Grace College of Pharmacy, Kodunthirapully, Palakkad, Kerala
2 Department of Pharmaceutical Chemistry, Grace College of Pharmacy, Kodunthirapully, Palakkad, Kerala
3 Department of Pharmacy Practice, KMCH College of Pharmacy, Coimbatore, Tamilnadu
     

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Dipeptidylpeptidase-4 is an enzyme involved in the inhibition of GLP-1 (Incretin), which is necessary for stimulation of insulin release. It is one of the enzyme targets for the treatment of type-2 diabetes mellitus. Ethanolic and aqueous extracts of Pergularia demia were investigated for activity using ellman method. Successive concentrations (50mg/ml, 100mg/ml, 200 mg/ml, 400 mg/ml, and 800 mg/ml) of ethanolic and aqueous extracts were prepared and investigated for their inhibitory activity towards dipeptidylpeptidase-4 as per Kojima et al., method. It was found that both extracts exhibited significant activity towards DPP-4 inhibition. Diprotein-A was used as standard and distilled water as control.

Keywords

Diabetes Mellitus, Dipeptidylpeptidase-4, Pergularia Demia (forsk), Enzymes
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  • Dipeptidylpeptidase-4 Inhibitory Activity of Pergularia Daemia (forsk) - An In-vitro Estimation

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Authors

Karthik Dhananjayan
Department of Pharmacology, Grace College of Pharmacy, Kodunthirapully, Palakkad, Kerala
Arunachalam Sumathy
Department of Pharmaceutical Chemistry, Grace College of Pharmacy, Kodunthirapully, Palakkad, Kerala
Sivanandy Palanisamy
Department of Pharmacy Practice, KMCH College of Pharmacy, Coimbatore, Tamilnadu

Abstract


Dipeptidylpeptidase-4 is an enzyme involved in the inhibition of GLP-1 (Incretin), which is necessary for stimulation of insulin release. It is one of the enzyme targets for the treatment of type-2 diabetes mellitus. Ethanolic and aqueous extracts of Pergularia demia were investigated for activity using ellman method. Successive concentrations (50mg/ml, 100mg/ml, 200 mg/ml, 400 mg/ml, and 800 mg/ml) of ethanolic and aqueous extracts were prepared and investigated for their inhibitory activity towards dipeptidylpeptidase-4 as per Kojima et al., method. It was found that both extracts exhibited significant activity towards DPP-4 inhibition. Diprotein-A was used as standard and distilled water as control.

Keywords


Diabetes Mellitus, Dipeptidylpeptidase-4, Pergularia Demia (forsk), Enzymes

References