Asian Journal of Research in Chemistry

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Extraction and Characterization of Hesperidine Present in Natural and Polyherbal formulation


Affiliations
1 Department of Pharmaceutical Analysis, JNTUA - Oil Technological Research Institute, Anantapur -515001, Andhra Pradesh, India
     

   Subscribe/Renew Journal


Hesperidine, a bioflavonoid, is an abundant and inexpensive by-product of Citrus cultivation. A deficiency of this substance in the diet has been linked with abnormal capillary leakiness as well as pain in the extremities causing aches, weakness and night leg cramps. No signs of toxicity have been observed with the normal intake of hesperidine or related compounds. Hesperidine was extracted from the dried peel of Citrus limetta which belongs to the family Rutaceae. An amount of 1kg air-dried and powdered peel was extracted to get 10gms of hesperidine which was a white brownish solid. Analysis of isolated hesperidine along with standard Hesperidine and marketed ayurvedic polyherbal formulation were identified by using IR spectroscopy, UV Spectroscopy, Thin Layer Chromatography (TLC) and UFLC. IR characterization showed that the standard, isolated and ayurvedic formulation were same. In UV spectroscopy all the components showed specific absorbance at 283.5nm. The Rf values for isolated, standard and marketed ayurvedic formulation were found to be 0.710, 0.708 and 0.702 respectively. A reversed phase UFLC method was also developed for determination and quantification of hesperidine. The method involved the use of a Phenomenex Luna 5u C18 column, a mixture of water-Acetonitrile-methanol (50:25:25 v/v/v) as a mobile phase in an isocratic mode at a flow rate of 1 ml/min and UV detection at 283.5 nm.

Keywords

Extraction, Characterization
Subscription Login to verify subscription
User
Notifications
Font Size


  • A. Garg, S. Garg, L. J. D. Zaneveld and A. K. Singla-Chemistry and Pharmacology of the Citrus Bioflavonoid Hesperidin- Phytotherapy Research Phytother. Res. 15, 655–669 (2001)

  • http://www.sciencelab.com/msds.php?msdsId=9924242

  • Kuhnau J. The flavonoids: A class of semi-essential food components: their role in human nutrition. World Res Nut Diet 1976; 24:117-91.

  • Cesarone M R, Laurora G, Ricci A, Belcaco G, Pomante P. Acute effects of Hydroxiethylrutosides on capillary filtration in normal volunteers, patients with various hypotension and in patients with diabetic micro angiopathy. J Vas Disease 1992; 21:76-80.

  • Bors W, Heller W, Michel C, Saran M, Flavonoids as antioxidants: Determination of radical scavenging efficiencies. Methods Enzymol., 1990;186:343-55.

  • Colerige Smith PO, Thomas P, Scurr JH, Dormandy JA. Causes of various ulceration, a new hypothesis. Br Med J 1980; 296:1726-7.

  • Clack W, Mackay E. Effect of flavonoid substances on histamine toxicity, anaphylactic shock and histamine enhanced capacity to dye. J Allergy 1950; 21:133-147.

  • Hertog MGL, Hollman PCH, Katan MB, KIomhout D. Intake of potentially anti-carcinogenic flavonoids and their determinantsin adults in the Netherlands. Nutr Cancer 1993; 20:21-9.

  • Mori A, Nishinoc, Enoki N, Tavata S. Cytotoxicity of plant flavonoids against Hela cells. Phytochemistry 1988; 27: 1017- 20.

  • Leposava A. Pavun , Jasmina M. Dimitric Markovic,Predrag T.Ðurðevic, Milena D. Jelikic-Stankov, Daniela B. Ðikanovic, Andrija R. Ciric And Dušan L. Malešev, Development And Validation Of A Spectrofluorimetric Method For The Determination Of Hesperidin In Human Plasma And Pharmaceutical Forms Journal Of The Serbian Chemical Society.,2012, 77 (0) 1–25.

  • Shrikant R. Kulkarni, Anil W Kharche, Analytical Method Development For Chiral Separations Of Fruity Flavonoids Using Rp-Hplc With Improved Selectivity And SensitivityJournal Of Engineering Research And Studies., 2010, 106-113.

  • Fatemeh Fathiazad, Jalil Afshar, An Investigation On Methylation Methods Of Hesperidin Daru., 2004, Volume 12, No.1.

  • Mi-Hee Chun, Eun Kyung Kim, Se Mi Yu, Myoung Sook Oh, Ki-Young Moon, Jee H. Jung, Jongki Hong, GC/MS combined with chemometrics methods for quality control of Schizonepeta tenuifolia Briq: Determination of essential oils Microchemical Journal., 2011, 274–281

  • R. Lobo, Richa Agrawal, Kirti.S.Prabhu, A.A. Shirwaikar, Mamtha Ballal,Nipun Dashora, Vijay Sodde, Annie Shirwaikar, Development and validation of a stability-indicating HPTLC method for analysis of Hesperidin in pharmaceutical dosage form Journal of Pharmacy Research., 2010, 3(7),1648-1651

  • Xiuling Li, Hongbin Xiao, Xinmiao Liang, Dazhuo Shi, Jiangang Liu, LC–MS/MS determination of naringin, hesperidin and neohesperidin in rat serum after orally administrating the decoction of Bulpleurum falcatum L. and Fractus aurantii Journal of Pharmaceutical and Biomedical Analysis., 2004, 159- 166

  • Muhammad Riaz, Nasir Rasool, Iftikhar Hussain Bukhari , Muhammad Shahid, Muhammad Zubair, Komal Rizwan and Umer Rashid, In Vitro Antimicrobial, Antioxidant, Cytotoxicity and GC-MS Analysis of Mazus goodenifolius Molecules., 2012, 17, 14275-14287

  • R. Ficarra, S. Tommasini, D. Raneri, M.L. Calabro, M.R. Di Bella, C. Rustichelli, M.C. Gamberini, P. Ficarra, Study of flavonoids/_-cyclodextrins inclusion complexes by NMR, FTIR, DSC, X-ray investigation Journal Of Pharmaceutical And Biomedical Analysis., 2002, 1005–1014.

  • http://kriemhild.uft.unibremen. de/nop/en/instructions/pdf/1023_en.pdf


Abstract Views: 336

PDF Views: 2




  • Extraction and Characterization of Hesperidine Present in Natural and Polyherbal formulation

Abstract Views: 336  |  PDF Views: 2

Authors

B Sandeep Kumar Naik
Department of Pharmaceutical Analysis, JNTUA - Oil Technological Research Institute, Anantapur -515001, Andhra Pradesh, India
N Duganath
Department of Pharmaceutical Analysis, JNTUA - Oil Technological Research Institute, Anantapur -515001, Andhra Pradesh, India
S Rubesh Kumar
Department of Pharmaceutical Analysis, JNTUA - Oil Technological Research Institute, Anantapur -515001, Andhra Pradesh, India
N Devanna
Department of Pharmaceutical Analysis, JNTUA - Oil Technological Research Institute, Anantapur -515001, Andhra Pradesh, India

Abstract


Hesperidine, a bioflavonoid, is an abundant and inexpensive by-product of Citrus cultivation. A deficiency of this substance in the diet has been linked with abnormal capillary leakiness as well as pain in the extremities causing aches, weakness and night leg cramps. No signs of toxicity have been observed with the normal intake of hesperidine or related compounds. Hesperidine was extracted from the dried peel of Citrus limetta which belongs to the family Rutaceae. An amount of 1kg air-dried and powdered peel was extracted to get 10gms of hesperidine which was a white brownish solid. Analysis of isolated hesperidine along with standard Hesperidine and marketed ayurvedic polyherbal formulation were identified by using IR spectroscopy, UV Spectroscopy, Thin Layer Chromatography (TLC) and UFLC. IR characterization showed that the standard, isolated and ayurvedic formulation were same. In UV spectroscopy all the components showed specific absorbance at 283.5nm. The Rf values for isolated, standard and marketed ayurvedic formulation were found to be 0.710, 0.708 and 0.702 respectively. A reversed phase UFLC method was also developed for determination and quantification of hesperidine. The method involved the use of a Phenomenex Luna 5u C18 column, a mixture of water-Acetonitrile-methanol (50:25:25 v/v/v) as a mobile phase in an isocratic mode at a flow rate of 1 ml/min and UV detection at 283.5 nm.

Keywords


Extraction, Characterization

References