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Development of New RP HPLC Method for the Simultaneous Estimation of Lopinavir and Ritonavir in API and in Tablet Dosage Form
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A New RP HPLC method was developed for simultaneous estimation of Lopinavir and Ritonavir. The separation was achieved by octadecyl column (C18) and Acetonitrile: water: Methanol (55: 30: 15 v/v/v), at flow rate 1 ml/ min. UV detection was carried out at 260 nm. The retention time of Lopinavir and Ritonavir is 10.82.±1 and 8.94 ±1 min respectively. The method has been validated for linearity, accuracy and precision. Linearity for Lopinavir was in the range of 50 - 250 μg/ml and for Ritonavir 10 - 50 μg/ml. The corelation coefficients were obtained 0.997 and 0.996 for Lopinavir and Ritonavir respectively, LOD was found to be 0.299μg/ml and 0.909μg/ml and LOQ was found to be 1.28μg/ml and 3.88μg/ml for lopinavir and for Ritonavir, respectively. The percentage recovery obtained for lopinavir and ritonavir were 101.72 and 103.8 respectively. The developed new method was found to be accurate, precise, selective and rapid for simultaneous estimation of lopinavir and ritonavir in tablets.
Keywords
Reverse Phase High Performance Liquid Chromatography (RP HPLC), Lopinavir, Ritonavir, Lopimune.
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