Zero Order, first Order and Second Order Derivative Spectrophotometric Methods for Determination of Buproprionhcl in Pharmaceutical formulation

Simple, fast and reliable derivative spectrophotometric methods were developed for determination of Buproprionhcl in bulk and pharmaceutical dosage forms. The solutions of standard and the sample were prepared in water. The quantitative determination of the drug was carried out using the zero order derivative values measured at 251 nm, the first order derivative values measured at 240 nm and the second order derivative values measured at 224 nm (n=6). Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of Buproprionhcl using 10-50 μg/ml (r<SUP>2</SUP> = 0.9955, r<SUP>2</SUP> = 0.9955 and r<SUP>2</SUP> = 0.996) for zero order, first order and second order derivative spectrophotometric method. All the proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. Developed spectrophotometric methods in this study are simple, accurate, precise, sensitive to assay of Buproprionhcl in tablets.