Asian Journal of Research in Chemistry

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Elemental Analysis and Anticariogenic Activity of Flowers of Calycopteris Floribunda, Humboldtia Brunonis and Kydia Calycina


Affiliations
1 Department of Biotechnology, UAS, GKVK, Bangalore-65, Karnataka, India
2 Department of Microbiology, SRNMN College of Applied Sciences, NES Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India
3 Department of Botany, Indira Gandi Government College, Sagar-577401, Karnataka, India
     

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The present study was conducted in order to estimate the content of elements and to determine the anticariogenic efficacy of flowers of Calycopteris floribunda (Roxb.) Poiret (Combretaceae), Humboldtia brunonis Wall (Caesalpiniaceae) and Kydia calycina Roxb. (Malvaceae). The major and minor elements in powdered flowers were estimated by Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES). Anticariogenic potential of petroleum ether, chloroform and methanol extracts of flowers was tested against 4 isolates of Streptococcus mutans by agar well diffusion method. Flower of K. calycina was found to contain high quantity of most of the elements estimated. The content of potassium and sodium was highest and least respectively among major elements. Among the minor elements, the content of iron and chromium was high and low respectively. The flower extracts were found to inhibit all four isolates of S. mutans. Among flowers, extracts of C. floribunda exhibited highest inhibition of test bacteria followed by K. calycina and H. brunonis. The flowers selected in the present study are shown to be a good source of various elements. Inhibition of cariogenic bacteria could be due to the presence of phytoconstituents present in flower extracts.

Keywords

Calycopteris Floribunda, Humboldtia Brunonis, Kydia Calycina, Elemental Analysis, ICP-OES, Anticariogenic, Agar well Diffusion
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  • Elemental Analysis and Anticariogenic Activity of Flowers of Calycopteris Floribunda, Humboldtia Brunonis and Kydia Calycina

Abstract Views: 462  |  PDF Views: 2

Authors

K.A. Ramesh Kumar
Department of Biotechnology, UAS, GKVK, Bangalore-65, Karnataka, India
G.M Pavithra
Department of Microbiology, SRNMN College of Applied Sciences, NES Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India
Syed Junaid
Department of Microbiology, SRNMN College of Applied Sciences, NES Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India
K.N. Rakesh
Department of Microbiology, SRNMN College of Applied Sciences, NES Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India
N. Dileep
Department of Microbiology, SRNMN College of Applied Sciences, NES Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India
Saba Siddiqua
Department of Microbiology, SRNMN College of Applied Sciences, NES Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India
Abhishiktha S. Naik
Department of Microbiology, SRNMN College of Applied Sciences, NES Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India
T. R Prashith Kekuda
Department of Microbiology, SRNMN College of Applied Sciences, NES Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India
K.S Vinayaka
Department of Botany, Indira Gandi Government College, Sagar-577401, Karnataka, India

Abstract


The present study was conducted in order to estimate the content of elements and to determine the anticariogenic efficacy of flowers of Calycopteris floribunda (Roxb.) Poiret (Combretaceae), Humboldtia brunonis Wall (Caesalpiniaceae) and Kydia calycina Roxb. (Malvaceae). The major and minor elements in powdered flowers were estimated by Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES). Anticariogenic potential of petroleum ether, chloroform and methanol extracts of flowers was tested against 4 isolates of Streptococcus mutans by agar well diffusion method. Flower of K. calycina was found to contain high quantity of most of the elements estimated. The content of potassium and sodium was highest and least respectively among major elements. Among the minor elements, the content of iron and chromium was high and low respectively. The flower extracts were found to inhibit all four isolates of S. mutans. Among flowers, extracts of C. floribunda exhibited highest inhibition of test bacteria followed by K. calycina and H. brunonis. The flowers selected in the present study are shown to be a good source of various elements. Inhibition of cariogenic bacteria could be due to the presence of phytoconstituents present in flower extracts.

Keywords


Calycopteris Floribunda, Humboldtia Brunonis, Kydia Calycina, Elemental Analysis, ICP-OES, Anticariogenic, Agar well Diffusion

References