Asian Journal of Research in Chemistry

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Reverse Phase High Performance Liquid Chromatographic Estimation of Atazanavir and Ritonavir in Pharmaceutical Dosage Form


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1 Department of Pharmaceutical Analysis and Quality Assurance, School of Pharmacy, Anurag Group of Institutions, Venkatapur (V), Ghatkesar (M), Rangareddy (D) AP
     

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A new simple, rapid, specific, accurate, precise and novel reverse phase High Performance Liquid Chromatography (RP-HPLC) method has been developed for the simultaneous estimation of atazanavir and ritonavir in the combined pharmaceutical dosage form. The chromatographic separation for Atazanavir and Ritonavir were achieved with mobile phase containing mixed phosphate buffer (pH 4.0) and acetonitrile (45:55 % v/v), Symmetry C18 (4.6 x 100mm, 3.5μm, Make: ACE) at 50C and UV detection at 237 nm. The compounds were eluted in the isocratic mode at a flow rate of 0.9 ml min-1. The retention times of atazavir 4.29 ± 0.09 min and ritonavir at 5.018± 0.09 min. The above method was validated in terms of linearity, accuracy, precision, LOD, LOQ etc. in accordance with ICH guidelines

Keywords

Atazanavir, Ritonavir, RP-HPLC, Validation
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  • Reverse Phase High Performance Liquid Chromatographic Estimation of Atazanavir and Ritonavir in Pharmaceutical Dosage Form

Abstract Views: 318  |  PDF Views: 4

Authors

J. Venkatesh
Department of Pharmaceutical Analysis and Quality Assurance, School of Pharmacy, Anurag Group of Institutions, Venkatapur (V), Ghatkesar (M), Rangareddy (D) AP
M. Singaiah Chowdary
Department of Pharmaceutical Analysis and Quality Assurance, School of Pharmacy, Anurag Group of Institutions, Venkatapur (V), Ghatkesar (M), Rangareddy (D) AP
D. Haritha
Department of Pharmaceutical Analysis and Quality Assurance, School of Pharmacy, Anurag Group of Institutions, Venkatapur (V), Ghatkesar (M), Rangareddy (D) AP
Anuroop
Department of Pharmaceutical Analysis and Quality Assurance, School of Pharmacy, Anurag Group of Institutions, Venkatapur (V), Ghatkesar (M), Rangareddy (D) AP
V. V. L. N. Prasad
Department of Pharmaceutical Analysis and Quality Assurance, School of Pharmacy, Anurag Group of Institutions, Venkatapur (V), Ghatkesar (M), Rangareddy (D) AP
V. Anjani Prasad Reddy
Department of Pharmaceutical Analysis and Quality Assurance, School of Pharmacy, Anurag Group of Institutions, Venkatapur (V), Ghatkesar (M), Rangareddy (D) AP

Abstract


A new simple, rapid, specific, accurate, precise and novel reverse phase High Performance Liquid Chromatography (RP-HPLC) method has been developed for the simultaneous estimation of atazanavir and ritonavir in the combined pharmaceutical dosage form. The chromatographic separation for Atazanavir and Ritonavir were achieved with mobile phase containing mixed phosphate buffer (pH 4.0) and acetonitrile (45:55 % v/v), Symmetry C18 (4.6 x 100mm, 3.5μm, Make: ACE) at 50C and UV detection at 237 nm. The compounds were eluted in the isocratic mode at a flow rate of 0.9 ml min-1. The retention times of atazavir 4.29 ± 0.09 min and ritonavir at 5.018± 0.09 min. The above method was validated in terms of linearity, accuracy, precision, LOD, LOQ etc. in accordance with ICH guidelines

Keywords


Atazanavir, Ritonavir, RP-HPLC, Validation

References