First Derivative Spectroscopic Method for Simultaneous Estimation of Edaravone and Argatroban in Synthetic Mixture

A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Edaravone and Argatroban in synthetic mixture using first order derivative zero-crossing method. Edaravone showed zero crossing point at 351.00 nm while Argatroban showed zero crossing point at 280.47 nm. The dA/dλ was measured at 280.47 nm for Edaravone and 351.00nm for Argatroban and calibration curves were plotted as dA/dλ versus concentration, respectively. The method was found to be linear (r2>0.998) in the range of 10-35μg/ml for Edaravone at 280.47 nm. The linear correlation was obtained (r2>0.999) in the range of 10-35 μg/ml for Argatroban at 351.0 nm. The limit of determination was 1.59μg/ml and 1.87μg/ml for Edaravone and Argatroban, respectively. The limit of quantification was 4.83 μg/ml and 5.68 μg/ml for Edaravone and Argatroban, respectively. The accuracy of these method were evaluated by recovery studies and good recovery result were obtained greater than 99% shows first order derivation zero crossing. The method was successfully applied for simultaneous determination of Edaravone and Argatroban in binary mixture.