Absorbance Correction Method for Simultaneous Estimation of Edaravone and Argatroban in Synthetic Mixture

A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Edaravone and Argatroban in synthetic mixture using Absorbance correction method. At 333 nm (λmax of Argatroban) Edaravone has zero absorbance so Argatroban is directly estimate at 333 nm. At 242 nm (λmax of Edaravone) both drugs have some absorbance so Edaravone is estimate at 242 nm using absorbance correction method.

The method was found to be linear (r2>0.992) in the range of 10-35μg/ml for Edaravone at 242 nm. The linear correlation was obtained (r2>0.998) in the range of 10-35 μg/ml for Argatroban at 333 nm. The limit of determination was 0.060 μg/ml and 0.208μg/ml for Edaravone and Argatroban, respectively. The limit of quantification was 0.184μg/ml and 0.631μg/ml for Edaravone and Argatroban, respectively. The accuracy of these method were evaluated by recovery studies and good recovery result were obtained greater than 99%. The method was successfully applied for simultaneous determination of Edaravone and Argatroban in binary mixture.