An Asian Journal of Soil Science

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Effect of Different Growth Regulator Combinations on in Vitro Callusing of Walnut (J. regia L.)


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1 Regional Research Station, (SKUAST-K), Wadura (J&K), India
     

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The present investigation on effect of different growth regulator combinations on in vitro callusing of walnut (J. regia L.) studies using MS medium was carried out in order to document the available genetic variability in walnut germplasm and to select elite walnut genotypes possessing superior attributes and quality traits. During the survey, data was recorded on one hundred fifty two (152) walnut trees growing in different areas of Kashmir valley. The study also involved establishment of response of elite walnut selections to different plant growth regulators in shoot morphogenesis. Woody species have been found to be far more difficult to clone in vitro than herbaceous plants. Poor response of the explants from mature woody species to in vitro manipulation is usually associated with the problem of browning and explant necrosis. The present studies were conducted on forced explants from three walnut selections (SKUAST 002, SKUAST 008, SKUAST 010). Murashiage and Skoog's basal medium supplemented with 0.3 mg/l-1 Benzylamino purine and 0.1 mg/l-1 indole-3-butyric acid the success varied between zero to 63.88 per cent. Data recorded in per cent was transformed to arc sin-1 values. Accessions revealed a non-significant difference in influencing the callusing of explant tissues after application of the growth regulators. The values for SKUA-002, SKUA-008 and SKUA-010 were 28.08, 25.25 and 28.52 corresponding 27.91, 24.68 and 28.55 per cent callusing, respectively.

Keywords

Walnut, Juglans regia L., Variability, Shoot Morphogenetic Response, Callusing of Walnut (J. regia L.).
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  • Anonymous (1984). Descriptor for walnut (Juglans regia spp.). International Plant Genetic Resource Institute, Rome Italy, pp. 28-40.

  • Cheema, G.S. and Mehra, P.N. (1982). Morphogensis in endosperm cultures. In : Plant tissue culture, 1982 (Ed. A. Fujiwara). Maruzen, Tokyo, pp 111-112.

  • Dodds, J.H. and Robert, L.W. (1982). Experiments in plant tissue culture. Cambridge University Press, London pp. 178.

  • Dolcet-Sanjuan, R., Calveria, E. and Alled, J. (1993). La micropropagation en clones de Juglans regia e hybrids de J. regia x. J. Nigra. Una Soluation Parcial a la Propagation Conal del nogal, 7: 114-119.

  • Driver, J.A. and Kuniyuki (1984). In vitro propagation of paradise walnut ischolar_mainstock. Hort. Sci., 19: 507-509.

  • Driver, J.A., Kuniyuki, A.H. and Hamber, P.C. (1984). Studies on in vitro culture of paradise walnut (Juglans regia L.). Hort. Sci., 19: 510-516.

  • Hu, C.Y. and Wang, P.J. (1983). Meristem shoot tip and bud cultures. In : Handbook of Plant Cell Cultures [Eds. D.A. Evans; W.R. Sharp, P.V. Ammirata and Y. Yamada]. Mac Millan Press, New York pp. 177-217.

  • Jay-Allemand, C.H., Peng, S., Capelro, P. and Cornu, D. (1993). Micropropagation of walnut hybrid tree : Some factors involved in ischolar_maining. Acta Horticulturae, 311: 117-124.

  • Mehra, A.S. and Mehra, P.N. (1974). Organogensis and plantlet formation in vitro in almond. Botanical Gazette, 135: 61-73.

  • Mele, E. and Messeguer, J. (1983). Clonal propagation of Corylus avellana L. in vitro. Proceedings of the International Plant Propagators Society (Italy).

  • Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol., 15: 473-497.

  • Perez, C., Fernander, B. and Rodriguez, R. (1983). In vitro plantlet regeneration through asexual embryogenesis in cotyledonary segments of Corylus avellana L. Plant Cell Reproduction, 2: 226-228.

  • Radojevic, L.R., Vujicic, R. and Neskoncm, M. (1975). Embryogenesis in tissue culture of Corylus avellana L.

  • Pflanezenphysiologia, 77: 33-41.

  • Rodriguez, R., Vazquez, M.A., Albuerne, M.A. and Sancher, T.R. (1983). Potencialidad miltipropgramable en laminas foliares de nogal. [c.f : Proc. 5th Revnion Nacional SEFV, Murcia, France p. 229-235].5.

  • Somers, P.W., Van Sambeek, J.W., Preece, J.E., Gaffney, G. and Myers, O. Jr. (1982). In vitro micropropagation of black walnut (Juglans nigra L.). Proceedings of the VII North American on Biological Sciences Workshop, pp. 26-28.

  • Street, H.E. (1977). Laboratory organization. In : Plant tissue and cell culture (Ed. H.E. Street). Blackwell Scientific Publications, Oxford. pp. 11-30.

  • Vieter and Vieiter (1980). The significance of allelopathy chestnut cultural systems. North America Growers Nut Association. Annual Report, 72: 117-134.

  • Anonymous (2007). Corylus colorna. Hazel, Turkish filbert, Turkish Hazelnut, Turkish tree hazel. [c.f.http//:zipcodezoo.com/plants/c/corylus-colornaasp.].


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  • Effect of Different Growth Regulator Combinations on in Vitro Callusing of Walnut (J. regia L.)

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Authors

Imtiyaz Ahmad Lone
Regional Research Station, (SKUAST-K), Wadura (J&K), India

Abstract


The present investigation on effect of different growth regulator combinations on in vitro callusing of walnut (J. regia L.) studies using MS medium was carried out in order to document the available genetic variability in walnut germplasm and to select elite walnut genotypes possessing superior attributes and quality traits. During the survey, data was recorded on one hundred fifty two (152) walnut trees growing in different areas of Kashmir valley. The study also involved establishment of response of elite walnut selections to different plant growth regulators in shoot morphogenesis. Woody species have been found to be far more difficult to clone in vitro than herbaceous plants. Poor response of the explants from mature woody species to in vitro manipulation is usually associated with the problem of browning and explant necrosis. The present studies were conducted on forced explants from three walnut selections (SKUAST 002, SKUAST 008, SKUAST 010). Murashiage and Skoog's basal medium supplemented with 0.3 mg/l-1 Benzylamino purine and 0.1 mg/l-1 indole-3-butyric acid the success varied between zero to 63.88 per cent. Data recorded in per cent was transformed to arc sin-1 values. Accessions revealed a non-significant difference in influencing the callusing of explant tissues after application of the growth regulators. The values for SKUA-002, SKUA-008 and SKUA-010 were 28.08, 25.25 and 28.52 corresponding 27.91, 24.68 and 28.55 per cent callusing, respectively.

Keywords


Walnut, Juglans regia L., Variability, Shoot Morphogenetic Response, Callusing of Walnut (J. regia L.).

References