Current Science

Open Access Open Access  Restricted Access Subscription Access

Vertebrate-Type Steroid Profile in Different Tissues of Wild and Endocrinologically Manipulated Female Brood Stocks of Penaeus monodon


Affiliations
1 Central Institute of Brackishwater Aquaculture, 75 Santhome High Road, RA Puram, Chennai 600 028, India
2 Centre for Climate Change Studies, Sathyabama University, Chennai 600 119, India, India
 

The endocrine mechanism regulating reproduction of tiger shrimp, Penaeus monodon, is a field of topical interest, which is to be addressed in detail as control of reproduction in captivity is conditional for domestication. In this study, the titres of estradiol and progesterone in hemolymph, ovary and hepatopancreas during various reproductive phases of females were quantified using ELISA. Circulating concentration of hemolymph estradiol and progesterone in the endocrinologically-induced (eyestalk ablated) females was also quantified. Additionally, the evidence for estradiol and progesterone receptors was provided using immunohistochemistry. Levels of progesterone in hemolymph and ovary were highest in the vitellogenic and ripe phases (P > 0.05). Conversely, in hepatopancreas significantly higher progesterone level was detected at immature phase (184.3 ± 2.3 pg/ml). Estradiol was highest in hemolymph (65.97 ± 0.8 pg/ml) and ovary (58.47 ± 0.7 pg/ml) at the early vitellogenic phase. In hepatopancreas, however, lowest level of estradiol was detected at the early vitellogenic phase (121 ± 0.38 pg/ml). Progesterone and estradiol receptor protein signals were predominantly found in the pre vitellogenic oocytes. These results suggest the possible involvement of vertebrate-type steroid in the reproductive maturation of P. monodon.

Keywords

Eyestalk Ablation, Immunohistochemistry, Penaeus monodon, Reproductive Maturation, Sex Steroids.
User
Notifications
Font Size

  • Edwards, D. P., Regulation of signal transduction pathways by estrogen and progesterone. Annu. Rev. Physiol., 2005, 67, 335–376.

  • Wierman, M. E., Sex steroid effects at target tissues: mechanisms of action. Adv. Physiol. Educ., 2007, 31(1), 26–33.

  • Wasson, K. M., Gower, B. A. and Watts, S. A., Responses of ovaries and testes of Lytechinus variegatus (Echinodermata: Echinoidea) to dietary administration of estradiol, progesterone and testosterone. Mar. Biol., 2000, 137(2), 245–255.

  • Lafont, R. and Mathieu, M., Steroids in aquatic invertebrates. Ecotoxicology, 2007, 16(1), 109–130.

  • Twan, W. H., Hwang, J. S. and Chang, C. F., Sex steroids in scleractinian coral, Euphyllia ancora: implication in mass spawning. Biol. Reprod., 2003, 68(6), 2255–2260.

  • Costantino, V., Dell’Aversano, C., Fattorusso, E. and Mangoni, A., Ecdysteroids from the Caribbean sponge Iotrochota birotulata. Steroids, 2000, 65(3), 138–142.

  • Escobedo, G., Larralde, C., Chavarria, A., Cerbón, M. A. and Morales-Montor, J., Molecular mechanisms involved in the differential effects of sex steroids on the reproduction and infectivity of Taenia crassiceps. J. Parasitol., 2004, 90(6), 1235–1244.

  • Garcia-Alonso, J., Hoeger, U. and Rebscher, N., Regulation of vitellogenesis in Nereis virens (Annelida: Polychaeta): effect of estradiol-17β on eleocytes. Comp. Biochem. Physiol. Part A, 2006, 143(1), 55–61.

  • Croll, R. P. and Wang, C., Possible roles of sex steroids in the control of reproduction in bivalve molluscs. Aquaculture, 2007, 272(1), 76–86.

  • Fingerman, M., Nagabhushanam, R. and Sarojini, R., Vertebratetype hormones in crustaceans: localization, identification and functional significance. Zool. Sci., 1993, 10(1), 13–29.

  • Panouse, J. B., Influence de l’ablation du pédoncule oculaire sur la croissance de l’ovaire chez la crevette Leander serratus. CR Acad. Sci. Paris, 1943, 217, 553–555.

  • Subramoniam, T., Mechanisms and control of vitellogenesis in crustaceans. Fish. Sci., 2011, 77(1), 1–21.

  • Laufer, H., Sagi, A., Ahl, J. S. B. and Homola, E., Methyl farnesoate appears to be a crustacean reproductive hormone. Invertebr. Reprod. Dev., 1992, 22(1–3), 17–19.

  • Tomy, S. et al., Serotonin induced changes in the expression of ovarian gene network in the Indian white shrimp, Penaeus indicus. Aquaculture, 2016, 452, 239–246.

  • Tsukimura, B., Crustacean vitellogenesis: its role in oocyte development. Am. Zool., 2001, 41(3), 465–476.

  • Gunamalai, V., Kirubagaran, R. and Subramoniam, T., Vertebrate steroids and the control of female reproduction in two decapod crustaceans, Emerita asiatica and Macrobrachium rosenbergii. Curr. Sci., 2006, 90(1), 119–123.

  • Merlin, J. et al., Induction of vitellogenesis and reproductive maturation in tiger shrimp, Penaeus monodon by 17β-estradiol and 17α-hydroxyprogesterone: in vivo and in vitro studies. Invertebrate Reprod. Develop., 2015, 59(3), 166–175.

  • Moore, R. K., Scott, A. P. and Collins, P. M., Circulating C-21 steroids in relation to reproductive condition of a viviparous marine teleost, Sebastes rastrelliger (grass rockfish). Gen. Comp. Endocrinol., 2000, 117(2), 268–280.

  • Quinitio, E. T., Hara, A., Yamauchi, K. and Nakao, S., Changes in the steroid hormone and vitellogenin levels during the gametogenic cycle of the giant tiger shrimp, Penaeus monodon. Comp. Biochem. Physiol. Part C, 1994, 109(1), 21–26.

  • Okumura, T. and Sakiyama, K., Hemolymph levels of vertebratetype steroid hormones in female kuruma prawn Marsupenaeus japonicus (Crustacea: Decapoda: Penaeidae) during natural reproductive cycle and induced ovarian development by eyestalk ablation. Fish. Sci., 2004, 70(3), 372–380.

  • Bell, T. A. and Lightner, D. V., A Handbook of Normal Penaeid Shrimp Histology, World Aquaculture Society, Baton Rouge, Louisiana, 1988.

  • Guzman, M. J., Norberg, B., Ramos, J., Mylonas, C. C. and Mananos, L. E., Vitellogenin, steroid plasma levels and spawning performance of cultured female Senegalese sole (Solea senegalensis). Gen. Comp. Endocrinol., 2001, 156(2), 285–297.

  • Brady, P., Elzur, A., Williams, R., Cummins, S. F. and Knibb, W., Gene expression profiling of cephalothorax and eyestalk in Penaeus monodon during ovarian maturation. Int. J. Biol. Sci., 2012, 8(3), 328–343.

  • Tan-Fermin, D. J. and Pudadera, A. R., Ovarian maturation stages of the wild giant tiger prawn, Penaeus monodon Fabricius. Aquaculture, 1989, 77(2), 229–242.

  • Munoz, G., Donghi, S. and Cerisola, H., Vitellogenesis in the crayfish Rhynchocinetes typus; role of hepatopancreas in lipid yolk biosynthesis. Cell Mol. Biol., 1990, 36, 531–536.

  • Warrier, S. R., Tirumalai, R. and Subramoniam, T., Occurrence of vertebrate steroids, estradiol 17β and progesterone in the reproducing females of the mud crab Scylla serrata. Comp. Biochem. Physiol. Part A, 2001, 30, 283–294.

  • Coccia, E., De Lisa, E., Di Cristo, C., Di Cosmo, A. and Paolucci, M., Effects of estradiol and progesterone on the reproduction of the freshwater crayfish Cherax albidus. Biol. Bull., 2010, 218(1), 36–47.


Abstract Views: 471

PDF Views: 129




  • Vertebrate-Type Steroid Profile in Different Tissues of Wild and Endocrinologically Manipulated Female Brood Stocks of Penaeus monodon

Abstract Views: 471  |  PDF Views: 129

Authors

J. R. Merlin
Central Institute of Brackishwater Aquaculture, 75 Santhome High Road, RA Puram, Chennai 600 028, India
D. L. Mohanlal
Central Institute of Brackishwater Aquaculture, 75 Santhome High Road, RA Puram, Chennai 600 028, India
C. P. Balasubramanian
Central Institute of Brackishwater Aquaculture, 75 Santhome High Road, RA Puram, Chennai 600 028, India
K. K. Vijayan
Central Institute of Brackishwater Aquaculture, 75 Santhome High Road, RA Puram, Chennai 600 028, India
T. Subramoniam
Centre for Climate Change Studies, Sathyabama University, Chennai 600 119, India, India
Sherly Tomy
Central Institute of Brackishwater Aquaculture, 75 Santhome High Road, RA Puram, Chennai 600 028, India
J. Syama Dayal
Central Institute of Brackishwater Aquaculture, 75 Santhome High Road, RA Puram, Chennai 600 028, India
C. Gopal
Central Institute of Brackishwater Aquaculture, 75 Santhome High Road, RA Puram, Chennai 600 028, India

Abstract


The endocrine mechanism regulating reproduction of tiger shrimp, Penaeus monodon, is a field of topical interest, which is to be addressed in detail as control of reproduction in captivity is conditional for domestication. In this study, the titres of estradiol and progesterone in hemolymph, ovary and hepatopancreas during various reproductive phases of females were quantified using ELISA. Circulating concentration of hemolymph estradiol and progesterone in the endocrinologically-induced (eyestalk ablated) females was also quantified. Additionally, the evidence for estradiol and progesterone receptors was provided using immunohistochemistry. Levels of progesterone in hemolymph and ovary were highest in the vitellogenic and ripe phases (P > 0.05). Conversely, in hepatopancreas significantly higher progesterone level was detected at immature phase (184.3 ± 2.3 pg/ml). Estradiol was highest in hemolymph (65.97 ± 0.8 pg/ml) and ovary (58.47 ± 0.7 pg/ml) at the early vitellogenic phase. In hepatopancreas, however, lowest level of estradiol was detected at the early vitellogenic phase (121 ± 0.38 pg/ml). Progesterone and estradiol receptor protein signals were predominantly found in the pre vitellogenic oocytes. These results suggest the possible involvement of vertebrate-type steroid in the reproductive maturation of P. monodon.

Keywords


Eyestalk Ablation, Immunohistochemistry, Penaeus monodon, Reproductive Maturation, Sex Steroids.

References





DOI: https://doi.org/10.18520/cs%2Fv111%2Fi7%2F1194-1200