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Complex Nature of Infection Associated with Yellow Vein Mosaic Disease in Bhendi (Abelmoschus esculentus)


Affiliations
1 Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai 625 021, India
 

Abelmoschus esculentus (Bhendi) is a traditional vege-table crop widely cultivated and consumed commonly in India. Yellow vein mosaic disease (YVMD) caused by Bhendi yellow vein mosaic virus (BYVMV) is a major constraint for Bhendi cultivation in India. To study the nature of infection in the field, we collected leaves which showed typical YVMD in Madurai (9 plants) and carried out rolling circle amplification (RCA) for plant #6. Intriguingly, the digestion of RCA product did not yield expected fragments. This sug-gests that there may be new viruses due to recombina-tion or mutation or mixed infection. However, on digesting the RCA product of plant #1 with SacI, monomers of 2.7 and 1.3 kb were released and each was cloned into the pOK12 vector. Sequenced RCA digested products showed mixed infection by BYVMV DNA A, Okra enation leaf curl virus (OELCuV) DNA A, beta, alphasatellite with the nanovirus origin of replication. Consequently, mixed infection was also confirmed by Southern hybridization and qPCR in all the analysed plant samples. In the mixed infection of BYVMV and OELCuV, we examined high level of OELCuV DNA A accumulation.

Keywords

Alphasatellite, BYVMV, Mixed Infection, OELCuV.
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  • Hanley-Bowdoin, L., Bejarano, E. R., Robertson, D. and Mansoor, S., Geminiviruses: masters at redirecting and reprogramming plant processes. Nat. Rev. Microbiol., 2013, 11, 777–788.

  • Rojas, M. R., Hagen, C., Lucas, W. J. and Gilbertson, R. L., Exploiting chinks in the plant’s armor: Evolution and emergence of geminiviruses. Annu. Rev. Phytopathol., 2005, 43, 361–394.

  • Adams, M. J., King, A. M. and Carstens, E. B., Ratification vote on taxonomic proposals to the international committee on taxono-my of viruses. Arch. Virol., 2013, 158, 2023–2030.

  • FAOSTAT, 2013, Production quantities by country. Food and agriculture organization of the United Nations; http://faostat3.fao.org/faostat-gateway/go/to/browse/Q/QC/E

  • Kulkarni, G. S., Mosaic and other related diseases of crops in the Bombay Presidency. Poona Agricul. College Mag., 1924, 16.

  • Pun, K. B. and Doraiswamy, S., Effect of age of okra plants on susceptibility to Okra yellow vein mosaic virus. Indian J. Virol., 1999, 15, 57–58.

  • Jose, J. and Usha, R., Bhendi yellow vein mosaic disease in India is caused by association of a DNA beta satellite with a be-gomovirus. Virology, 2003, 305, 310–317.

  • Gopal, P., Pravin Kumar, P., Sinilal, B., Jose, J., Kasin Yadunan-dam, A. and Usha, R., Differential roles of C4 and betaC1 in me-diating suppression of post-transcriptional gene silencing: evidence for transactivation by the C2 of Bhendi yellow vein mo-saic virus, a monopartite begomovirus. Virus Res., 2007, 123, 9–18.

  • Chandran, S. A., Levy, Y., Mett, A., Belausov, E., Ramakrishnan, U. and Gafni, Y., Mapping of functional region conferring nuclear localization and karyopherin alpha-binding activity of the C2 pro-tein of Bhendi yellow vein mosaic virus. J. Gen. Virol., 2012, 93, 1367–1374.

  • Kumar, P. P., Usha, R., Zrachya, A., Levy, Y., Spanov, H. and Gafni, Y., Protein-protein interactions and nuclear trafficking of coat protein and betaC1 protein associated with bhendi yellow vein mosaic disease. Virus Res., 2006, 122, 127–136.

  • Varma, A. and Malathi, V. G., Emerging geminivirus problems: a serious threat to crop production. Ann. Appl. Biol., 2003, 142, 145–164.

  • Nawaz-Ul-Rehman, M. S. and Fauquet, C. M., Evolution of geminiviruses and their satellites. FEBS Lett., 2009, 583, 1825–1832.

  • Venkataravanappa, V., Lakshminarayana Reddy, C. N., Devaraju, A., Jalali, S. and Krishna Reddy, M., Association of a recombinant Cotton leaf curl Bangalore virus with yellow vein and leaf curl disease of okra in India. Indian J. Virol., 2013, 24, 188–198.

  • Venkataravanappa, V., Lakshminarayana Reddy, C. N., Jalali, S. and Krishna Reddy, M., Molecular characterization of a new spe-cies of begomovirus associated with yellow vein mosaic of bhendi (Okra) in Bhubhaneswar, India. Eur. J Plant Pathol., 2013, 136, 811–822.

  • Rashmi, R., Biswanath, M. and Indranil, D., Diverse and recombi-nant begomoviruses and various satellites are associated with Bhendi yellow vein mosaic disease of okra in India. J. Plant Bio-chem. Biotech., 2015, 24, 470–475.

  • Jose, J. and Usha, R., Extraction of Geminiviral DNA from a highly mucilaginous plant (Abelmoschus esculentus). Plant Mol. Biol. Rep., 2000, 18, 349–355.

  • Southern, E. M., Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol., 1975, 98, 503–517.

  • Xie, Y., Wu, P., Liu, P., Gong, H. and Zhou, X., Characterization of alphasatellites associated with monopartite begomovirus/betasatellite complexes in Yunnan, China. Virol. J., 2010, 7, 178–187.

  • Chandran, S. A. et al., First report of an alphasatellite associated with Okra enation leaf curl virus. Virus Genes, 2013, 46, 585–587.

  • Umaharan, P., Padidam, M., Phelps, R. H., Beachy, R. N. and Fauquet, C. M., Distribution and diversity of geminiviruses in Trinidad and Tobago. Phytopathology, 1998, 88, 1262–1268.

  • Padidam, M., Sawyer, S. and Fauquet, C. M., Possible emergence of new geminiviruses by frequent recombination. Virology, 1999, 265, 218–225.

  • Sanz, A. I., Fraile, A., Garcia-Arenal, F., Zhou, X., Robinson, D. J., Khalid, S., Butt, T. and Harrison, B. D., Multiple infection, recombination and genome relationships among begomovirus iso-lates found in cotton and other plants in Pakistan. J. Gen. Virol., 2000, 81, 1839–1849.

  • Ribeiro, S. G. et al., Distribution and genetic diversity of tomato-infecting begomoviruses in Brazil. Arch. Virol., 2003, 148, 281–295.

  • Suresh, K., Babasaheb, B., Meera, K., Prem, R. and Radhamani, A., Mixed Groundnut bud necrosis virus and Okra yellow vein mosaic virus infection of Okra in India. Plant Health Prog., 2012; http://dx.doi.org/10.1094/PHP-2012-1023-01-RS

  • Rentería-Canett, I., Xoconostle-Cazares, B., Ruiz-Medrano, R. and Rivera-Bustamante, R. F., Geminivirus mixed infection on pepper plants: Synergistic interaction between PHYVV and pepGMV. Virology J., 2011, 8, 104–116.

  • Fondong, V. N., Pita, J. S., Rey, M. E., de Kochko, A., Beachy, R. N. and Fauquet, C. M., Evidence of synergism between African cassava mosaic virus and a new double-recombinant geminivirus infecting cassava in Cameroon. J. Gen. Virol., 2000, 81, 287–297.

  • Singh, A. K., Chattopadhyay, B. and Chakraborty, S., Biology and interactions of two distinct monopartite begomoviruses and beta-satellites associated with radish leaf curl disease in India. Virol. J., 2012, 9, 43–60.

  • Ala-Poikela, M., Svensson, E., Rojas, A., Horko, T., Paulin, L., Valkonen, J. P. T. and Kvarnheden, A., Genetic diversity and mixed infections of begomoviruses infecting tomato, pepper and cucurbit crops in Nicaragua. Plant Pathol., 2005, 54, 448–459.

  • Kuan, C. P., Huang, H. C., Chang, C. C. and Lu, Y. L., Taqman real-time pcr for detection and quantiation of squash leaf curl virus in cucurbits. J. Virol. Meth., 2012, 179, 367–372.

  • Abrahamian, P. E., Seblani, R., Sobh, H. and Abou-Jawdah, Y., Detection and quantitation of two cucurbit criniviruses in mixed infection by real-time rt-pcr. J. Virol. Meth., 2013, 193, 320–326.


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  • Complex Nature of Infection Associated with Yellow Vein Mosaic Disease in Bhendi (Abelmoschus esculentus)

Abstract Views: 417  |  PDF Views: 135

Authors

P. Priyavathi
Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai 625 021, India
V. Kavitha
Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai 625 021, India
P. Gopal
Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai 625 021, India

Abstract


Abelmoschus esculentus (Bhendi) is a traditional vege-table crop widely cultivated and consumed commonly in India. Yellow vein mosaic disease (YVMD) caused by Bhendi yellow vein mosaic virus (BYVMV) is a major constraint for Bhendi cultivation in India. To study the nature of infection in the field, we collected leaves which showed typical YVMD in Madurai (9 plants) and carried out rolling circle amplification (RCA) for plant #6. Intriguingly, the digestion of RCA product did not yield expected fragments. This sug-gests that there may be new viruses due to recombina-tion or mutation or mixed infection. However, on digesting the RCA product of plant #1 with SacI, monomers of 2.7 and 1.3 kb were released and each was cloned into the pOK12 vector. Sequenced RCA digested products showed mixed infection by BYVMV DNA A, Okra enation leaf curl virus (OELCuV) DNA A, beta, alphasatellite with the nanovirus origin of replication. Consequently, mixed infection was also confirmed by Southern hybridization and qPCR in all the analysed plant samples. In the mixed infection of BYVMV and OELCuV, we examined high level of OELCuV DNA A accumulation.

Keywords


Alphasatellite, BYVMV, Mixed Infection, OELCuV.

References





DOI: https://doi.org/10.18520/cs%2Fv111%2Fi9%2F1511-1515