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Rumen Virome:An Assessment of Viral Communities and their Functions in the Rumen of an Indian Buffalo


Affiliations
1 Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 380 001, India
 

Viruses play a key role in compensating bacterial population in any ecosystem of the planet. Rumen, a highly diverse ecosystem, is still under-explored for viral communities and their metabolic capabilities. We carried out shotgun sequencing of enriched viral particles from rumen fluid collected from an Indian buffalo. The study revealed that well-assembled con-tigs of Newbler and Velvet got majority of assignments to virus domain that further revealed Caudovirales as a major order. A majority of the Firmicutes bacterio-phages were found in the study, which also confirm the presence of conserved domains such as peptidases against Firmicutes phages.

Keywords

Bacteriophage, Contigs, Gene Prediction, Peptidase, Virome.
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  • Rumen Virome:An Assessment of Viral Communities and their Functions in the Rumen of an Indian Buffalo

Abstract Views: 341  |  PDF Views: 159

Authors

Nidhi R. Parmar
Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 380 001, India
Subhash J. Jakhesara
Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 380 001, India
Amitbikram Mohapatra
Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 380 001, India
Chaitanya G. Joshi
Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 380 001, India

Abstract


Viruses play a key role in compensating bacterial population in any ecosystem of the planet. Rumen, a highly diverse ecosystem, is still under-explored for viral communities and their metabolic capabilities. We carried out shotgun sequencing of enriched viral particles from rumen fluid collected from an Indian buffalo. The study revealed that well-assembled con-tigs of Newbler and Velvet got majority of assignments to virus domain that further revealed Caudovirales as a major order. A majority of the Firmicutes bacterio-phages were found in the study, which also confirm the presence of conserved domains such as peptidases against Firmicutes phages.

Keywords


Bacteriophage, Contigs, Gene Prediction, Peptidase, Virome.



DOI: https://doi.org/10.18520/cs%2Fv111%2Fi5%2F919-925