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Effect of Environmental Temperature on the Isolation of Mycobacterium tuberculosis from Sputum Samples Stored in Cetylpyridinium Chloride


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1 Regional Medical Research Centre, P.O. South Eastern Railways Complex, Bhubaneswar 751 023, India
 

Isolation of Mycobacterium tuberculosis from sputa of suspected tuberculosis patients is essential for treatment initiation and drug resistance studies. In case the sputa cannot be processed within 72 h, they can be stored at room temperature with the addition of cetylpyridinium chloride (CPC) for longer periods (up to two weeks) without compromising on the quality of mycobacteria isolation. It has been recommended that CPC containing sputum should be stored at temperatures above 20°C as CPC crystallizes at low temperatures and may become inactive as a preservative.
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  • Effect of Environmental Temperature on the Isolation of Mycobacterium tuberculosis from Sputum Samples Stored in Cetylpyridinium Chloride

Abstract Views: 369  |  PDF Views: 118

Authors

Dasarathi Das
Regional Medical Research Centre, P.O. South Eastern Railways Complex, Bhubaneswar 751 023, India
Bhagirathi Dwibedi
Regional Medical Research Centre, P.O. South Eastern Railways Complex, Bhubaneswar 751 023, India

Abstract


Isolation of Mycobacterium tuberculosis from sputa of suspected tuberculosis patients is essential for treatment initiation and drug resistance studies. In case the sputa cannot be processed within 72 h, they can be stored at room temperature with the addition of cetylpyridinium chloride (CPC) for longer periods (up to two weeks) without compromising on the quality of mycobacteria isolation. It has been recommended that CPC containing sputum should be stored at temperatures above 20°C as CPC crystallizes at low temperatures and may become inactive as a preservative.

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DOI: https://doi.org/10.18520/cs%2Fv111%2Fi12%2F1911-1912