Current Science

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Isolation of Mycobacterium tuberculosis from sputa of suspected tuberculosis patients is essential for treatment initiation and drug resistance studies. In case the sputa cannot be processed within 72 h, they can be stored at room temperature with the addition of cetylpyridinium chloride (CPC) for longer periods (up to two weeks) without compromising on the quality of mycobacteria isolation. It has been recommended that CPC containing sputum should be stored at temperatures above 20°C as CPC crystallizes at low temperatures and may become inactive as a preservative.
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