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In silico Characterization of Ferritin-1 Chloroplast Targeting Peptide Encoding Sequence in Chickpea and Pigeonpea


Affiliations
1 Division of Plant Biotechnology, Indian Institute of Pulses Research, Kanpur 208 024, India
 

Chloroplast is an important organelle and a hub of photosynthetic reactions that occur in all plants. Majority of the chloroplast proteins are encoded by the nucleus and then transported into the chloroplast. The chloroplast transport is aided by leader sequence, chloroplast targeting peptide (cTP) that directs the proteins into the chloroplast. We identified the genes encoding chloroplast targeting proteins from chickpea (Cicer arietinum) and pigeonpea (Cajanus cajan and Cajanus cajanifolius) genomes. All the sequences were aligned, conserved motifs were identified and their phylogeny was deciphered. In this study, structural and functional features of chloroplast transit peptides of Ferritin-1 and its putative role in chloroplast tar-geting have been examined. The study would impart better understanding of targeting heterologous pro-teins to chloroplasts in pulses.

Keywords

Chloroplast Targeting Peptide, Domains, Ferritin-1, Motifs, Transgenes.
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  • In silico Characterization of Ferritin-1 Chloroplast Targeting Peptide Encoding Sequence in Chickpea and Pigeonpea

Abstract Views: 388  |  PDF Views: 129

Authors

Poornima K. Narayana
Division of Plant Biotechnology, Indian Institute of Pulses Research, Kanpur 208 024, India
Alok Das
Division of Plant Biotechnology, Indian Institute of Pulses Research, Kanpur 208 024, India
Narendra P. Singh
Division of Plant Biotechnology, Indian Institute of Pulses Research, Kanpur 208 024, India

Abstract


Chloroplast is an important organelle and a hub of photosynthetic reactions that occur in all plants. Majority of the chloroplast proteins are encoded by the nucleus and then transported into the chloroplast. The chloroplast transport is aided by leader sequence, chloroplast targeting peptide (cTP) that directs the proteins into the chloroplast. We identified the genes encoding chloroplast targeting proteins from chickpea (Cicer arietinum) and pigeonpea (Cajanus cajan and Cajanus cajanifolius) genomes. All the sequences were aligned, conserved motifs were identified and their phylogeny was deciphered. In this study, structural and functional features of chloroplast transit peptides of Ferritin-1 and its putative role in chloroplast tar-geting have been examined. The study would impart better understanding of targeting heterologous pro-teins to chloroplasts in pulses.

Keywords


Chloroplast Targeting Peptide, Domains, Ferritin-1, Motifs, Transgenes.



DOI: https://doi.org/10.18520/cs%2Fv111%2Fi11%2F1838-1841