Antiproliferative Effects of Fluorine Substitute 3,5-di-tert-butylphenol Bearing Schiff Bases Using CFSE-Based Cell Proliferation Assay

The determination of antiproliferative properties of compounds on tumour cells is important for assessment of their efficacy in cancer treatment. CFSE labelled K562 cells were incubated with doxorubicin and ortho- or para-fluorosubstitute Schiff bases (compounds 1 and 2 respectively). CFSE intensities were analysed using flow cytometry. K562 cells treated with doxorubicin resulted in homogeneous high intensity fluorescence after 96 h of incubation. Schiff bases exhibited antiproliferative effects, but lower than doxorubicin. Our results reveal that CFSE assay can be used for determining in vitro anti proliferative features of anticancer drugs and/or compounds from herbal or chemical sources.