Current Science

Open Access Open Access  Restricted Access Subscription Access

A Simple Egg Membrane Model for Understanding Diffusion Characteristics of Nanoparticles and Amino Acids


Affiliations
1 Department of Biophysics, University of Mumbai, Vidyanagari, Santacruz (E), Mumbai 400 098, India
 

This study reports the passive diffusion (in vitro) of silver nanoparticles (SNPs) and those of the amino acids tryptophan, phenylalanine, tyrosine across a biological membrane model. The experiments were carried out under physiological conditions at pH 7.4. Chicken egg shell outer membrane model was used to study the passive diffusion of the above materials. Passive diffusion was performed against and towards gravitation for 24 and 48 h. Fick's first law of diffusion was adopted for quantification of diffusion coefficient, permeability constant and diffusion rate. The egg shell membrane was characterized using scanning electron microscopy. The SNPs were synthesized by chemical degradation method and characterized by UV-visible spectroscopy and dynamic light scattering. An average size of nanoparticles obtained was 62 nm. The diffusion rates of amino acids were higher than those of SNPs. However, they were enhanced in their presence. Permeability coefficient and diffusion coefficient were higher for amino acids than SNPs. The possible mechanisms have been explained on the basis of molecular properties.

Keywords

Amino Acids, Chicken Egg Shell Membrane, Diffusion Rate, Permeability Constant, Silver Nanoparticles.
User
Notifications
Font Size

  • Korkusuz, H. et al., Transferrin-coated gadolinium nanoparticles as MRI contrast agent. Mol. Imaging Biol., 2013, 15(2), 148–154.

  • Asadishad, B., Vossoughi, M. and Alemzadeh. Folate-receptortargeted delivery of doxorubicin using polyethylene glycolfunctionalized gold nanoparticles. Ind. Eng. Chem. Res., 2010; 49(4), 1958–1963.

  • Wang, T., Bai, J., Jiang, X. and Nienhus, G. V., Cellular uptake of nanoparticles by membrane penetration: a study of combining confocal microscopy with FTIR spectrochemistry. ACS Nano, 2012, 6, 1251–1259.

  • Rothen-Rutishauser, B. M., Schurch, S., Haenni, B., Kapp, N. and Gehr, P., Interaction of fine particles and nanoparticles with red blood cells visualized with advanced microscopic techniques. Environ. Sci. Technol., 2006, 40, 4353–4359.

  • Labhshetwar, V., Song, C., Humphery, W., Shebuski, R. and Levy, R. J., Arterial uptake of biodegradable nanoparticles: effect of surface modification. J. Pharm. Sci., 1998, 87, 1229–1234.

  • Chithrani, B. D., Ghazani, A. A. and Chan, W. C. W., Determining the size and shape dependence of gold nanoparticles uptake into mammalian cells. Nano Lett., 2006, 6, 662–668.

  • Li, Y., Yue, T., Yang, K. and Zhang, X., Molecular modeling of the relationship between nanoparticle shape anisotropy and endocytosis kinetics. Biomaterials, 2012, 33, 4965–4973.

  • Kim, J. A., Aberg, C., Salvati, A. and Dawson, K. A., Role of cell cycle on the cellular uptake and dilution of nanoparticles in cell population. Nat. Nanotechnol., 2012, 7, 62–68.

  • Jiang, X., Musyanovych, A., Rocker, C., Landfester, K., Mailander, V. and Nienhaus, G. U., Specific effects of surface carboxyl groups on anionic polysterene particle in their interaction with mesenchymal stem cell. Nanoscale, 2011, 3, 2028–2035.

  • Jiang, X., Weise, S., Hafner, M., Rocker, C., Zhang, F., Parkar, N. J. and Nienhaus, G. U., Quantitative analysis of the protein corona on FePt nanoparticles formed by transferring binding. J. R. Soc. Interface, 2010, 7, S5–S13.

  • Cho, E. C., Zhang, Q. and Xia, Y., The effect of sedimentation and diffusion on cellular uptake of gold nanoparticles. Nat. Nanotechnol., 2011, 6, 385–391.

  • Schultz, S. G., Basic Principle of Membrane Transport, Cambridge University Press, Cambridge, 1980.

  • Steen-Knudsen, O., Biological Membranes, Theory of Transport, Potentials and Electric Impulses, Cambridge University Press, Cambridge, 2012.

  • Twist, J. N. and Zatz, J. L., Influence of solvents on paraben permeation through idealized skin model membrane. J Sac. Cosmet. Chem., 1986, 37, 429–444.

  • Olivella, M., Debattisa, N. B. and Pappano, N. B., Salicyclic acid permeation: a comparative study with different vehicles and membranes. Biocell, 2006, 30(2), 321–324.

  • Petro, E. et al., In vitro and in vivo evaluation of drug release from semisolid dosage form. Pharmazie, 2011, 66, 936–941.

  • Christensen, J. M., Chang, M. C., Le, H. and Ehab Bendas. Hydrocortisone diffusion through synthetic membrane, mouse skin and EpidermTM cultured skin. Arch. Drug Info., 2011, 4, 10–21.

  • Brink, P. R. and Ramanan, S. V., A model for diffusion of fluorescent probes in the septate giant axon of earthworm. Biophys. J., 1985, 48, 299–309.

  • Burley, R. W. and Vedehra, D. V., The egg shell and shell membrane: properties and synthesis. In Avian Egg, Chemistry and Biology, John Wiley, New York, 1989, pp 25–64.

  • Lammie, D., Bain, M. M. and Wess, T. J., Microfocus X-ray scattering investigation of egg shell nanotexture. J. Synchrot. Rad., 2005, 12, 721–726.

  • Lee, P. C. and Meisel, D., Adsorption of surface enhanced Raman of dyes on silver and gold sols. J. Phys. Chem., 1982, 86, 3391–3395.

  • Jessy, M., Dongre, P. M. and Kothari, D. C., Study of interaction of silver nanoparticles with bovine serum albumin using fluorescence spectroscopy. J. Fluor., 2011, 21; doi:101007/s10895-011-1922-3.

  • Simon, P. C. M., Ultrastructure of hen egg-shell and its physiological interpretation, PhD Thesis, Landbouwhoge School, Wageningen, Netherlands, Centre for Agricultural Publishing and Documentation, 1971.

  • Tan, C. K., Chen, T. W., Chen, H. L. and Ng, L. S., A scanning and transmission electron microscopic study of the membranes of chicken egg. Histol. Histopathol., 1992, 7, 339–345.

  • Nys, Y. and Gautron, J., In Bioactive Egg Compounds, Ch. 15, Springer, Berlin, Heidelberg, 2007, pp. 99–102.

  • Zamyatinin, A. A., Protein volume solution. Prog. Biophys. Mol. Biol., 1972, 24, 107–123.

  • Jessy, M., Sivakami, S. and Dongre, P. M., Bioactivity of albumins bound to silver nanoparticles. Protein J., 2014, 33(3), 258–266.


Abstract Views: 360

PDF Views: 104




  • A Simple Egg Membrane Model for Understanding Diffusion Characteristics of Nanoparticles and Amino Acids

Abstract Views: 360  |  PDF Views: 104

Authors

Deepika Tari
Department of Biophysics, University of Mumbai, Vidyanagari, Santacruz (E), Mumbai 400 098, India
Shalaka Haryan
Department of Biophysics, University of Mumbai, Vidyanagari, Santacruz (E), Mumbai 400 098, India
Kaustubh Patankar
Department of Biophysics, University of Mumbai, Vidyanagari, Santacruz (E), Mumbai 400 098, India
Vinod Jaiswal
Department of Biophysics, University of Mumbai, Vidyanagari, Santacruz (E), Mumbai 400 098, India
Mahesh Samant
Department of Biophysics, University of Mumbai, Vidyanagari, Santacruz (E), Mumbai 400 098, India
S. Sivakami
Department of Biophysics, University of Mumbai, Vidyanagari, Santacruz (E), Mumbai 400 098, India
P. M. Dongre
Department of Biophysics, University of Mumbai, Vidyanagari, Santacruz (E), Mumbai 400 098, India

Abstract


This study reports the passive diffusion (in vitro) of silver nanoparticles (SNPs) and those of the amino acids tryptophan, phenylalanine, tyrosine across a biological membrane model. The experiments were carried out under physiological conditions at pH 7.4. Chicken egg shell outer membrane model was used to study the passive diffusion of the above materials. Passive diffusion was performed against and towards gravitation for 24 and 48 h. Fick's first law of diffusion was adopted for quantification of diffusion coefficient, permeability constant and diffusion rate. The egg shell membrane was characterized using scanning electron microscopy. The SNPs were synthesized by chemical degradation method and characterized by UV-visible spectroscopy and dynamic light scattering. An average size of nanoparticles obtained was 62 nm. The diffusion rates of amino acids were higher than those of SNPs. However, they were enhanced in their presence. Permeability coefficient and diffusion coefficient were higher for amino acids than SNPs. The possible mechanisms have been explained on the basis of molecular properties.

Keywords


Amino Acids, Chicken Egg Shell Membrane, Diffusion Rate, Permeability Constant, Silver Nanoparticles.

References





DOI: https://doi.org/10.18520/cs%2Fv112%2Fi07%2F1574-1578