Current Science

Open Access Open Access  Restricted Access Subscription Access

Interactive Effects of EDTA and Oxalic Acid on Chromium Uptake, Translocation and Photosynthetic Attributes in Indian Mustard (Brassica juncea L. var. Varuna)


Affiliations
1 Department of Botany, University of Delhi, Delhi 110 007, India
 

The accumulation and toxicity of chromium (Cr) in Indian mustard (Brassica juncea L.) under ethylene diamine tetraacetic acid (EDTA) and oxalic acid (OA) as chelating agents was studied. Plants were exposed to Cr(VI) and chelating agents in four experimental set-ups as Cr(VI), Cr(VI) + EDTA (1 : 1), Cr(VI) + OA (1 : 1) and Cr(VI) + EDTA + OA (1 : 1 : 1), where each set-up comprised of 0, 6.25, 12.5 and 25.0 mg of Cr(VI) and/or chelating agents in 250 g of soil. Results conferred that EDTA augmented bio-concentration factor in all the three doses of Cr(VI), OA considerably increased translocation factor in all the treatments, including control, and the combined application of the two chelates escalated both the aforesaid factors. Moreover, these chelating agents help in ameliorating Cr(VI) toxicity asserted by low degree of lipid peroxidation, insubstantial damage in ischolar_main and shoot length, fresh and dry biomass, chlorophyll and leaf gas exchange parameters. Besides, plants showed a robust detoxification mechanism primarily by significant (P < 0.05) production of reduced glutathione and phytochelatins among other enzymatic and nonenzymatic antioxidants under these chelating agents. The present findings suggest that Indian mustard could be used as a potential phytoremediator of Cr(VI) under the combined application of EDTA and OA.

Keywords

Brassica juncea L., Chromium Uptake, Chelating Agents, Photosynthesis.
User
Notifications
Font Size

  • Singh, H. P., Mahajan, P., Kaur, S., Batish, D. R. and Kohli, R. K., Chromium toxicity and tolerance in plants. Environ. Chem. Lett., 2013, 11, 229–254.

  • Seth, C. S. et al., Phytoextraction of toxic metals: a central role for glutathione. Plant Cell Environ., 2012, 35, 334–346.

  • Ogunleyel, J. H., Dagari, M. S., Balogun, O. L. and Maigari, A., Effect of oxalic acid on Cr+3 ion uptake, accumulation and oxidative stress by sorrel (Hibiscus sabdariffa L.) seedlings in hydroponic solution. Int. J. Chem. Mater. Environ. Res., 2016, 3, 49–55.

  • Li, H. et al., Enhanced efficiency of cadmium removal by Boehmeria nivea (L.) Gaud. in the presence of exogenous citric and oxalic acids. J. Environ. Sci., 2014, 26, 2508–2516.

  • APHA, AWWA and WEF, Standard Methods for the Examination of Water and Wastewater, American Public Health Association, Washington (DC), 2005, 21st edn.

  • Hiscox, J. D. and Israelstam, G. F., A method for extraction of chlorophyll from leaf tissue without maceration. Can. J. Bot., 1979, 57, 1332–1334.

  • Heath, R. L. and Packer, L., Photo-peroxidation in isolated chloroplasts: kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys., 1968, 125, 189–198.

  • Beyer, W. F. and Fridovich, I., Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal. Chem., 1987, 161, 559–566.

  • Aebi, H., Catalase. In Methods of Enzymatic Analysis (ed. Bergmeyer, H. U.), Verlag Chemie, Weinheim, 1974, p. 680.

  • Nakano, Y. and Asada, K., Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol., 1981, 22, 867–880.

  • Hemeda, H. M. and Klein, B. P., Effects of naturally occurring antioxidants on peroxidase activity of vegetable extracts. Egypt. J. Food Sci., 1990, 55, 184–185.

  • Foyer, C. H. and Halliwell, B., The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism. Planta, 1976, 133, 21–25.

  • Gaitonde, M. K., Spectrophotometric method for the direct determination of cysteine in the presence of other naturally occurring amino acids. Biochem. J., 1967, 104, 627–633.

  • Ellman, G. L., Tissue sulfhydryl groups. Arch. Biochem. Biophys., 1959, 82, 70–77.

  • Anderson, M. E., Determination of glutathione and glutathione disulphide in biological samples. Methods Enzymol., 1985, 113, 548–555.

  • Hsiao, K. H., Kao, P. H. and Hseu, Z. Y., Effects of chelators on chromium and nickel uptake by Brassica juncea on serpentinemine tailings for phytoextraction. J. Hazard. Mater., 2007, 148, 366–376.

  • Foyer, C. H. and Shigeoka, S., Understanding oxidative stress and antioxidant functions to enhance photosynthesis. Plant Physiol., 2011, 155, 93–100.

  • Zhang, Y. Xu, S., Yang, S. and Chen, Y., Salicylic acid alleviates cadmium-induced inhibition of growth and photosynthesis through upregulating antioxidant defense system in two melon cultivars (Cucumis melo L.). Protoplasma, 2015, 252, 911–924.

  • Jean, L., Bordas, F., Moussard, C. G., Vernay, P., Hitmi, A. and Bollinger, J. C., Effect of citric acid and EDTA on chromium and nickel uptake and translocation by Datura innoxia. Environ. Pollut., 2008, 153, 555–563.

  • Lim, B., Meyer, A. J. and Cobbett, C. S., Development of glutathionedeficient embryos in Arabidopsis is influenced by the maternal level of glutathione. Plant Biol., 2011, 13, 693–697.

  • Srivastava, S., Tripathi, R. D. and Dwivedi, U. N., Synthesis of phytochelatins and modulation of antioxidants in response to cadmium stress in Cuscuta reflexa – an angiospermic parasite. J. Plant Physiol., 2004, 161, 665–674.

  • Foyer, C. H. and Noctor, G., Stress-triggered redox signalling: what's in pROSpect? Plant Cell Environ., 2016, 39, 951–964.

  • Hernández, L. E., Plata, J. S., Palmero, M. B. M., Gil, S. C., Cáceres, M. L. F., Villasante, C. O. and Escobar, C., Contribution of glutathione to the control of cellular redox homeostasis under toxic metal and metalloid stress. J. Exp. Bot., 2015, 66, 2901–2911.

  • Nazi, N., Ali, A., Javad, H. and Shahram, P., Phenylalanin ammonialyase activity, total phenolic and flavonoid content in flowers, leaves, hulls and kernels of three pistachio (Pistacia vera L.) cultivars. Am.-Eur. J. Agric. Environ. Sci., 2012, 12, 807–814.


Abstract Views: 304

PDF Views: 93




  • Interactive Effects of EDTA and Oxalic Acid on Chromium Uptake, Translocation and Photosynthetic Attributes in Indian Mustard (Brassica juncea L. var. Varuna)

Abstract Views: 304  |  PDF Views: 93

Authors

Durga Singh
Department of Botany, University of Delhi, Delhi 110 007, India
Ashish Agnihotri
Department of Botany, University of Delhi, Delhi 110 007, India
Chandra Shekhar Seth
Department of Botany, University of Delhi, Delhi 110 007, India

Abstract


The accumulation and toxicity of chromium (Cr) in Indian mustard (Brassica juncea L.) under ethylene diamine tetraacetic acid (EDTA) and oxalic acid (OA) as chelating agents was studied. Plants were exposed to Cr(VI) and chelating agents in four experimental set-ups as Cr(VI), Cr(VI) + EDTA (1 : 1), Cr(VI) + OA (1 : 1) and Cr(VI) + EDTA + OA (1 : 1 : 1), where each set-up comprised of 0, 6.25, 12.5 and 25.0 mg of Cr(VI) and/or chelating agents in 250 g of soil. Results conferred that EDTA augmented bio-concentration factor in all the three doses of Cr(VI), OA considerably increased translocation factor in all the treatments, including control, and the combined application of the two chelates escalated both the aforesaid factors. Moreover, these chelating agents help in ameliorating Cr(VI) toxicity asserted by low degree of lipid peroxidation, insubstantial damage in ischolar_main and shoot length, fresh and dry biomass, chlorophyll and leaf gas exchange parameters. Besides, plants showed a robust detoxification mechanism primarily by significant (P < 0.05) production of reduced glutathione and phytochelatins among other enzymatic and nonenzymatic antioxidants under these chelating agents. The present findings suggest that Indian mustard could be used as a potential phytoremediator of Cr(VI) under the combined application of EDTA and OA.

Keywords


Brassica juncea L., Chromium Uptake, Chelating Agents, Photosynthesis.

References





DOI: https://doi.org/10.18520/cs%2Fv112%2Fi10%2F2034-2042