Parthenium hysterophorus is considered as one of the most devastating and hazardous weed; abundantly available in several parts of the world, it is utilized as a substrate for the production of polyhydroxyalkanoates (PHA). Bacterial strain Bacillus aerophilus, isolated from oil contaminated soil, was studied for its potential to accumulate PHA. Utilizing this cheap substrate, the highest yield of PHA content obtained was 5.4 g/l PHA with 11.92 g/l cell biomass. PHA produced was extracted using sodium hypochlorite method and the polymer synthesized was characterized as polyhydroxybutyrate (P3 (HB)) by Fourier transform infrared spectroscopy and nuclear magnetic resonance analysis.
Keywords
Biopolymer, Bacillus aerophilus, Biomass, Parthenium hysterophorus, P3 (HB).
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