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Histological Localization of Fungal Endophytes in Healthy Tissues of Adhatoda vasica Nees


Affiliations
1 Department of Biosciences, Manipal University, Jaipur 303 007, India
2 Biotechnology Laboratory, Department of Botany, University of Rajasthan, Jaipur 302 055, India
 

A research protocol has been developed to locate endophytic fungi present inside the healthy leaves and stems of Adhatoda vasica using the microtome technique. The surface-sterilized explants after staining with lactophenol cotton blue showed the presence of endophytic fungi in intercellular spaces of ground and dermal tissues. In transverse sections of leaf at 100 magnification, dense blue colonies of endophytic fungi in epidermis and mesophyll region were recorded, while sprinkled colonies were seen in vascular bundles. In transverse sections of stem at 100 magnification, dense colonies of endophytic fungi in phloem and slightly lesser in epidermal region were observed. Scattered colonies were also recorded in xylem and cortex tissues. The fungal colonies were also present in intracellular and extracellular regions of both explants. Further studies will be required to identify these fungal isolates through pure culture on PDA plates to explore their biosynthetic pathway.

Keywords

Adhatoda vasica, Endophytic Fungi, Histological Localization, Microtome.
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  • Histological Localization of Fungal Endophytes in Healthy Tissues of Adhatoda vasica Nees

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Authors

Yash Mishra
Department of Biosciences, Manipal University, Jaipur 303 007, India
Amla Batra
Biotechnology Laboratory, Department of Botany, University of Rajasthan, Jaipur 302 055, India
Madan Mohan Sharma
Department of Biosciences, Manipal University, Jaipur 303 007, India

Abstract


A research protocol has been developed to locate endophytic fungi present inside the healthy leaves and stems of Adhatoda vasica using the microtome technique. The surface-sterilized explants after staining with lactophenol cotton blue showed the presence of endophytic fungi in intercellular spaces of ground and dermal tissues. In transverse sections of leaf at 100 magnification, dense blue colonies of endophytic fungi in epidermis and mesophyll region were recorded, while sprinkled colonies were seen in vascular bundles. In transverse sections of stem at 100 magnification, dense colonies of endophytic fungi in phloem and slightly lesser in epidermal region were observed. Scattered colonies were also recorded in xylem and cortex tissues. The fungal colonies were also present in intracellular and extracellular regions of both explants. Further studies will be required to identify these fungal isolates through pure culture on PDA plates to explore their biosynthetic pathway.

Keywords


Adhatoda vasica, Endophytic Fungi, Histological Localization, Microtome.

References





DOI: https://doi.org/10.18520/cs%2Fv112%2Fi10%2F2112-2115