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Screening of Autochthonous Intestinal Microbiota as Candidate Probiotics Isolated from Four Freshwater Teleosts


Affiliations
1 Fisheries Laboratory, Department of Zoology, Visva-Bharati University, Santiniketan 731 235, India
2 Department of Zoology, University of Burdwan, Golap Bag, Burdwan 713 104, India
 

In this study altogether 109 autochthonous gut bacteria were screened from 4 fish species (Labeo rohita, Labeo bata, Catla catla and Puntius javanicus), of which 13 isolates displayed antagonism to 4 fish potent pathogens, namely Aeromonas salmonicida, Aeromonas hydrophila, Pseudomonas fluorescens and Aeromonas sobria. Eight promising isolates were further evaluated for extracellular enzyme production, non-hemolytic activity, bile tolerance and identified by 16S rRNA sequencing. Strains CCF7 (identified as Bacillus sp.), CCH9 and PJH1 (identified as two strains of Bacillus amyloliquefaciens) exhibited high score in antagonism assay and fulfilled other probiotic criteria, including safety aspects. However, application of these probiotics in aquaculture industries requires in vivo experiments and other information like immune modulating efficiency and binding ability on gut.

Keywords

Antagonism, Bacillus Species, Fish Gut Bacteria, Athogen, Probiotics.
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  • Screening of Autochthonous Intestinal Microbiota as Candidate Probiotics Isolated from Four Freshwater Teleosts

Abstract Views: 387  |  PDF Views: 126

Authors

Ankita Nandi
Fisheries Laboratory, Department of Zoology, Visva-Bharati University, Santiniketan 731 235, India
Goutam Banerjee
Fisheries Laboratory, Department of Zoology, Visva-Bharati University, Santiniketan 731 235, India
Suhas Kumar Dan
Fisheries Laboratory, Department of Zoology, Visva-Bharati University, Santiniketan 731 235, India
Pinki Ghosh
Fisheries Laboratory, Department of Zoology, Visva-Bharati University, Santiniketan 731 235, India
Koushik Ghosh
Department of Zoology, University of Burdwan, Golap Bag, Burdwan 713 104, India
Arun Kumar Ray
Fisheries Laboratory, Department of Zoology, Visva-Bharati University, Santiniketan 731 235, India

Abstract


In this study altogether 109 autochthonous gut bacteria were screened from 4 fish species (Labeo rohita, Labeo bata, Catla catla and Puntius javanicus), of which 13 isolates displayed antagonism to 4 fish potent pathogens, namely Aeromonas salmonicida, Aeromonas hydrophila, Pseudomonas fluorescens and Aeromonas sobria. Eight promising isolates were further evaluated for extracellular enzyme production, non-hemolytic activity, bile tolerance and identified by 16S rRNA sequencing. Strains CCF7 (identified as Bacillus sp.), CCH9 and PJH1 (identified as two strains of Bacillus amyloliquefaciens) exhibited high score in antagonism assay and fulfilled other probiotic criteria, including safety aspects. However, application of these probiotics in aquaculture industries requires in vivo experiments and other information like immune modulating efficiency and binding ability on gut.

Keywords


Antagonism, Bacillus Species, Fish Gut Bacteria, Athogen, Probiotics.

References





DOI: https://doi.org/10.18520/cs%2Fv113%2Fi04%2F767-773