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Callus-Mediated Organogenesis in Lilium polyphyllum D. Don ex Royle:A Critically Endangered Astavarga Plant


Affiliations
1 Botanical Survey of India, Northern Regional Centre, Dehradun 248 195, India
2 Department of Botany, Delhi University, New Delhi 110 021, India
 

Lilium polyphyllum D. Don ex Royle (Liliaceae) is a critically endangered herbaceous perennial, commonly known as white lily or Ksheer kakoli. Bulbs of the plant are of immense medicinal use and have a stringent and anti-inflammatory properties. Over exploitation of the species from the wild and degradation of habitats are posing threats to its existence. In the present study, a protocol was standardized for micropropagation and mass multiplication of the species from scale leaves. Callusing was induced in basal MS medium containing 2,4-D (6.78 μM) and BAP(4.4 μM), where maximum effect (95.32%) was recorded. Maximum shooting (97.45%) was found in the calluses when shifted to MS medium fortified with BAP (4.4 μM), NAA (0.53 μM) and GA3 (20 ppm) with an average of 19.2 shoots/per culture. The well developed in vitro regenerated shoots were shifted to the ischolar_maining medium and 100% ischolar_maining was achieved in half-strength MS basal medium enriched with IBA(9.8 μM). The in vitro regenerated plant lets were shifted to a glasshouse for acclimatization and finally transferred to the open environment with 85% success.

Keywords

Callusing, Lilium polyphyllum, Micro-Propagation, 0rganogenesis.
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  • Callus-Mediated Organogenesis in Lilium polyphyllum D. Don ex Royle:A Critically Endangered Astavarga Plant

Abstract Views: 434  |  PDF Views: 135

Authors

G. S. Panwar
Botanical Survey of India, Northern Regional Centre, Dehradun 248 195, India
P. L. Uniyal
Department of Botany, Delhi University, New Delhi 110 021, India

Abstract


Lilium polyphyllum D. Don ex Royle (Liliaceae) is a critically endangered herbaceous perennial, commonly known as white lily or Ksheer kakoli. Bulbs of the plant are of immense medicinal use and have a stringent and anti-inflammatory properties. Over exploitation of the species from the wild and degradation of habitats are posing threats to its existence. In the present study, a protocol was standardized for micropropagation and mass multiplication of the species from scale leaves. Callusing was induced in basal MS medium containing 2,4-D (6.78 μM) and BAP(4.4 μM), where maximum effect (95.32%) was recorded. Maximum shooting (97.45%) was found in the calluses when shifted to MS medium fortified with BAP (4.4 μM), NAA (0.53 μM) and GA3 (20 ppm) with an average of 19.2 shoots/per culture. The well developed in vitro regenerated shoots were shifted to the ischolar_maining medium and 100% ischolar_maining was achieved in half-strength MS basal medium enriched with IBA(9.8 μM). The in vitro regenerated plant lets were shifted to a glasshouse for acclimatization and finally transferred to the open environment with 85% success.

Keywords


Callusing, Lilium polyphyllum, Micro-Propagation, 0rganogenesis.

References





DOI: https://doi.org/10.18520/cs%2Fv113%2Fi05%2F946-951