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In vitro Micropropagation, Total Phenolic Content and Comparative Antioxidant Activity of Different Extracts of Sesbania grandiflora (L.) Pers.


Affiliations
1 Department of Biotechnology, Jeppiaar Engineering College, Old Mamallapuram Road, Rajiv Gandhi Salai, Chennai 600 119, India
2 Centre for Ecological Genomics and Wildlife Conservation, Department of Zoology, University of Johannesburg, P.O. Box 524, Auckland Park-2006, South Africa
3 Department of Pharmaceutical Sciences, Tshwane University of Technology, Pretoria 0001, South Africa
4 Department of Phytochemistry, Uttarakhand Ayurved University, Harrawala, Dehradun 248 001, India
 

Sesbania grandiflora (L.) Pers. is a common traditional medicinal plant used in bronchitis, anaemia,headache, fever, ophthalmia, nasal catarrh, leprosy, inflammation, gout and rheumatism. The present study aimed to assess plant regeneration and plantlets development in vitro using explants of S. grandiflora together with the estimation of total phenolic contentand antioxidative activity of various extracts obtained from the plant. Murashige and Skoog (MS) basal medium added with different concentrations of plant growth regulators (PGRs) was used for plant tissue culture, whereas ferric reducing antioxidant power(FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH)assays were used to evaluate the antioxidant potential of different extracts of the plant. In the presence of 6-benzylaminopurine (BAP; 0.1 mg/l), the highest level(85.41%) of seed germination was achieved while the highest callus formation (96.6%) was recorded with 2,4-dichlorophenoxyacetic acid (2,4-D; 0.5 mg/l). Inaddition, the highest shoot induction, shoot formation and shoot elongation were observed with BAP(0.1 mg/l), indole-3-butyric acid (3 mg/l) and naphthaleacetic acid + BAP (0.4 + 0.2 mg/l) respectively.The extract of dried calluses showed highest contents of proline (110.94 mg/g), phenol (16.42 mg/g) and flavonoid (22.22 mg/g), and also highest antioxidant potential with FRAP and DPPH assays. From the present study, we may conclude that the MS basal medium supplemented with PGRs is effective for the commercial production of S. grandiflora.

Keywords

Antioxidant Activity, In vitro Micropropagation, Phenolic Content, Plant Growth Regulators, Sesbania grandiflora.
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  • In vitro Micropropagation, Total Phenolic Content and Comparative Antioxidant Activity of Different Extracts of Sesbania grandiflora (L.) Pers.

Abstract Views: 430  |  PDF Views: 148

Authors

Krishnamoorthy Vinothini
Department of Biotechnology, Jeppiaar Engineering College, Old Mamallapuram Road, Rajiv Gandhi Salai, Chennai 600 119, India
Masilamani Sri Devi
Department of Biotechnology, Jeppiaar Engineering College, Old Mamallapuram Road, Rajiv Gandhi Salai, Chennai 600 119, India
Veronica Shalini
Department of Biotechnology, Jeppiaar Engineering College, Old Mamallapuram Road, Rajiv Gandhi Salai, Chennai 600 119, India
Sudharshan Sekar
Centre for Ecological Genomics and Wildlife Conservation, Department of Zoology, University of Johannesburg, P.O. Box 524, Auckland Park-2006, South Africa
Ruchi Badoni Semwal
Department of Pharmaceutical Sciences, Tshwane University of Technology, Pretoria 0001, South Africa
Pandian Arjun
Department of Pharmaceutical Sciences, Tshwane University of Technology, Pretoria 0001, South Africa
Deepak Kumar Semwal
Department of Phytochemistry, Uttarakhand Ayurved University, Harrawala, Dehradun 248 001, India

Abstract


Sesbania grandiflora (L.) Pers. is a common traditional medicinal plant used in bronchitis, anaemia,headache, fever, ophthalmia, nasal catarrh, leprosy, inflammation, gout and rheumatism. The present study aimed to assess plant regeneration and plantlets development in vitro using explants of S. grandiflora together with the estimation of total phenolic contentand antioxidative activity of various extracts obtained from the plant. Murashige and Skoog (MS) basal medium added with different concentrations of plant growth regulators (PGRs) was used for plant tissue culture, whereas ferric reducing antioxidant power(FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH)assays were used to evaluate the antioxidant potential of different extracts of the plant. In the presence of 6-benzylaminopurine (BAP; 0.1 mg/l), the highest level(85.41%) of seed germination was achieved while the highest callus formation (96.6%) was recorded with 2,4-dichlorophenoxyacetic acid (2,4-D; 0.5 mg/l). Inaddition, the highest shoot induction, shoot formation and shoot elongation were observed with BAP(0.1 mg/l), indole-3-butyric acid (3 mg/l) and naphthaleacetic acid + BAP (0.4 + 0.2 mg/l) respectively.The extract of dried calluses showed highest contents of proline (110.94 mg/g), phenol (16.42 mg/g) and flavonoid (22.22 mg/g), and also highest antioxidant potential with FRAP and DPPH assays. From the present study, we may conclude that the MS basal medium supplemented with PGRs is effective for the commercial production of S. grandiflora.

Keywords


Antioxidant Activity, In vitro Micropropagation, Phenolic Content, Plant Growth Regulators, Sesbania grandiflora.

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DOI: https://doi.org/10.18520/cs%2Fv113%2Fi06%2F1142-1147